Kaur Jagdeep, Sidhu Shabir, Chopra Kanwaljit, Khan M U
a Department of Research Innovations and Consultancy , IKG Punjab Technical University , Kapurthala , Punjab , India.
b Sri Sai College of Pharmacy, Badhani , Pathankot , Punjab , India.
Pharm Biol. 2016 Dec;54(12):2951-2959. doi: 10.1080/13880209.2016.1195848. Epub 2016 Jun 23.
Calendula officinalis L. (Asteraceae) has been traditionally used in treating inflammation of internal organs, gastrointestinal tract ulcers and wound healing.
The present study investigates the effect of ethanol extract (95%) of Calendula officinalis flowers in l-arginine induced acute necrotizing pancreatitis in rats.
Rats were divided into four groups: normal control, l-arginine control, Calendula officinalis extract (COE) treated and melatonin treated (positive control), which were further divided into subgroups (24 h, day 3 and 14) according to time points. Two injections of l-arginine 2 g/kg i.p. at 1 h intervals were administered in l-arginine control, COE and melatonin-treated groups to produce acute necrotizing pancreatitis. Biochemical parameters [serum amylase, lipase, pancreatic amylase, nucleic acid content, total proteins, transforming growth factor-β1 (TGF-β1), collagen content, lipid peroxidation, reduced glutathione and nitrite/nitrate] and histopathological studies were carried out.
COE treatment (400 mg/kg p.o.) was found to be beneficial. This was evidenced by significantly lowered histopathological scores (2 at day 14). Nucleic acid content (DNA 21.1 and RNA 5.44 mg/g pancreas), total proteins (0.66 mg/mL pancreas) and pancreatic amylase (1031.3 100 SU/g pancreas) were significantly improved. Marked reduction in pancreatic oxidative and nitrosative stress; collagen (122 μmoles/100 mg pancreas) and TGF-β1 (118.56 pg/mL) levels were noted. Results obtained were comparable to those of positive control.
The beneficial effect of COE may be attributed to its antioxidant, antinitrosative and antifibrotic actions. Hence, the study concludes that COE promotes spontaneous repair and regeneration of the pancreas.
金盏花（菊科）传统上用于治疗内脏炎症、胃肠道溃疡及伤口愈合。
本研究探讨金盏花花乙醇提取物（95%）对L-精氨酸诱导的大鼠急性坏死性胰腺炎的影响。
将大鼠分为四组：正常对照组、L-精氨酸对照组、金盏花提取物（COE）治疗组和褪黑素治疗组（阳性对照组），并根据时间点进一步分为亚组（24小时、第3天和第14天）。在L-精氨酸对照组、COE组和褪黑素治疗组中，每隔1小时腹腔注射两次2 g/kg的L-精氨酸，以诱导急性坏死性胰腺炎。进行生化参数[血清淀粉酶、脂肪酶、胰腺淀粉酶、核酸含量、总蛋白、转化生长因子-β1（TGF-β1）、胶原蛋白含量、脂质过氧化、还原型谷胱甘肽和亚硝酸盐/硝酸盐]及组织病理学研究。
发现COE治疗（口服400 mg/kg）有益。这在第14天组织病理学评分显著降低（2分）中得到证明。核酸含量（胰腺DNA 21.1和RNA 5.44 mg/g）、总蛋白（胰腺0.66 mg/mL）和胰腺淀粉酶（1031.3 100 SU/g胰腺）显著改善。胰腺氧化应激和亚硝化应激明显降低；胶原蛋白（122 μmol/100 mg胰腺）和TGF-β1（118.56 pg/mL）水平也有所降低。所得结果与阳性对照组相当。
COE的有益作用可能归因于其抗氧化、抗亚硝化和抗纤维化作用。因此，该研究得出结论，COE可促进胰腺的自发修复和再生。
