Mosabbir Abdullah Al, Truong Kevin
Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, M5S 3G9, Canada.
Edward S. Rogers Sr. Department of Electrical and Computer Engineering, University of Toronto, 10 King's College Circle, Toronto, ON, M5S 3G4, Canada.
Biotechnol Lett. 2016 Oct;38(10):1715-21. doi: 10.1007/s10529-016-2164-6. Epub 2016 Jun 24.
To use HIV-1 based lentivirus components to produce gene integration and the formation of a stable cell line in the packaging cell line without viral infection.
A co-transfection of a Human Embryonic Kidney (HEK) 293 packaging cell line with Gag-pol (GP) and a transfer vector, without the envelope vector, produces a stable cell line after 2 weeks of selection. Furthermore, a matrix protein deficient GP in the packaging vector enhances this integration. This supports that, in theory, unexported lentiviral cores produced within the packaging cell can infect itself without requiring the release of any lentiviral particles.
If the packaging cell is also the target cell, then gene integration leading to a stable cell line can be accomplished without viral particle infection.
利用基于HIV-1的慢病毒组件在包装细胞系中实现基因整合并形成稳定细胞系，而无需病毒感染。
将人胚肾（HEK）293包装细胞系与Gag-pol（GP）和转移载体共转染，不使用包膜载体，经过2周筛选后产生了稳定细胞系。此外，包装载体中基质蛋白缺陷的GP增强了这种整合。这支持了理论上，在包装细胞内产生的未输出的慢病毒核心可以在不释放任何慢病毒颗粒的情况下感染自身。
如果包装细胞也是靶细胞，那么无需病毒颗粒感染即可实现导致稳定细胞系的基因整合。
