Kalaiyarasu Semmannan, Bhatia Sandeep, Mishra Niranjan, Sood Richa, Kumar Manoj, SenthilKumar D, Bhat Sushant, Dass Prakash M
ICAR- National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh 462 022, India.
ICAR- National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh 462 022, India.
Cytokine. 2016 Sep;85:140-7. doi: 10.1016/j.cyto.2016.06.022. Epub 2016 Jun 23.
The study was designed to characterize and compare chicken bone marrow and peripheral blood monocyte derived dendritic cells (chBM-DC and chMoDC) and to evaluate inflammatory cytokine and chemokine alterations in response upon LPS stimulation. Typical morphology was observed in DCs from 48h of culture using recombinant chicken GM-CSF and IL-4. Maturation of DCs with LPS (1μg/ml) showed significant up regulation of mRNA of surface markers (CD40, CD80, CD83, CD86, MHC-II and DC-LAMP (CD208)), pro-inflammatory cytokines (IL-1β, IL-6, TNF-α (LITAF)), iNOS, chemokine CXCli2 and TLRs4 and 15. Basal level of TLR1 mRNA expression was higher followed by TLR15 in both DCs irrespective of their origin. Expression of iNOS and CXCLi2 mRNA in mature DCs of both origins were higher than other surface molecules and cytokines studied. Hence, its level of expression can also be used as an additional maturation marker for LPS induced chicken dendritic cell maturation along with CD83 and CD40. LPS matured DCs of both origins upregulated IL-12 and IFN-γ. Based on CD40 and CD83 mRNA expression, it was observed that LPS induced the maturation in both DCs, but chMoDCs responded better in expression of surface markers and inflammatory mediator genes.
本研究旨在表征和比较鸡骨髓和外周血单核细胞衍生的树突状细胞（chBM-DC和chMoDC），并评估脂多糖（LPS）刺激后炎症细胞因子和趋化因子的变化。使用重组鸡粒细胞巨噬细胞集落刺激因子（GM-CSF）和白细胞介素-4（IL-4）培养48小时后，观察到树突状细胞具有典型形态。用LPS（1μg/ml）使树突状细胞成熟，结果显示表面标志物（CD40、CD80、CD83、CD86、主要组织相容性复合体II类分子（MHC-II）和树突状细胞相关膜蛋白（DC-LAMP，即CD208））、促炎细胞因子（IL-1β、IL-6、肿瘤坏死因子-α（LITAF））、诱导型一氧化氮合酶（iNOS）、趋化因子CXCli2以及Toll样受体4和15的mRNA显著上调。无论其来源如何，两种树突状细胞中Toll样受体1（TLR1）mRNA的基础表达水平较高，其次是TLR15。两种来源的成熟树突状细胞中iNOS和CXCLi2 mRNA的表达均高于所研究的其他表面分子和细胞因子。因此，其表达水平也可作为LPS诱导鸡树突状细胞成熟的额外成熟标志物，与CD83和CD40一起使用。两种来源的LPS成熟树突状细胞均上调了IL-12和干扰素-γ（IFN-γ）。基于CD40和CD83 mRNA表达，观察到LPS诱导了两种树突状细胞的成熟，但chMoDCs在表面标志物和炎症介质基因的表达方面反应更好。
