CD4cre转基因在B6·Cg-Tg(Cd4-cre)1Cwi小鼠中的染色体定位。
Chromosomal localisation of the CD4cre transgene in B6·Cg-Tg(Cd4-cre)1Cwi mice.
作者信息
Westendorf Kerstin, Durek Pawel, Ayew Samia, Mashreghi Mir-Farzin, Radbruch Andreas
机构信息
Deutsches Rheuma-Forschungszentrum Berlin, Chariteplatz 1, 10117 Berlin, Germany.
Deutsches Rheuma-Forschungszentrum Berlin, Chariteplatz 1, 10117 Berlin, Germany.
出版信息
J Immunol Methods. 2016 Sep;436:54-7. doi: 10.1016/j.jim.2016.06.005. Epub 2016 Jun 23.
The B6·Cg-Tg(Cd4-cre)1Cwi line expresses CRE recombinase under the control of the promoter and regulatory elements of the Cd4 gene. Here we show that CRE recombinase expression reduces the number and frequencies of CD4 positive subsets in a dose-dependent manner and localize the integration site of the transgenic construct to position 60335693-60341285 (qD) of chromosome 3. The insert contains at least 15 complete sequential copies of the transgenic construct. Based on this information we describe a novel PCR assay for genetic typing of transgenic mice.
B6·Cg-Tg(Cd4-cre)1Cwi品系在Cd4基因的启动子和调控元件控制下表达CRE重组酶。我们在此表明，CRE重组酶的表达以剂量依赖方式减少CD4阳性亚群的数量和频率，并将转基因构建体的整合位点定位到3号染色体的60335693 - 60341285（qD）位置。该插入片段包含转基因构建体的至少15个完整连续拷贝。基于此信息，我们描述了一种用于转基因小鼠基因分型的新型PCR检测方法。
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