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降钙素减轻骨关节炎实验大鼠模型中的软骨退变和痛觉过敏：软骨细胞中的 TGF-β 作用。

Calcitonin attenuates cartilage degeneration and nociception in an experimental rat model of osteoarthritis: role of TGF-β in chondrocytes.

机构信息

Department of Marine Biotechnology &Resources, and Center for Translational Biopharmaceuticals, National Sun Yat-Sen University, Kaohsiung, Taiwan.

Department of Early Childhood Education, National Pintung University, Taiwan.

出版信息

Sci Rep. 2016 Jun 27;6:28862. doi: 10.1038/srep28862.

DOI:10.1038/srep28862
PMID:27345362
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4921823/
Abstract

We investigated the role of the calcitonin (Miacalcin) in the progression of osteoarthritis (OA) and in nociceptive behavior in an experimental rat model of OA and osteoporosis. OA was induced by anterior cruciate ligament transection (ACLT) of the right knee and by bilateral ovariectomy (OVX) in Wistar rats. Nociceptive behaviors (secondary mechanical allodynia and weight-bearing distribution of the hind paws) were analyzed prior to surgery and every week, beginning at 12 weeks after surgery, up to 20 weeks. At 20 weeks, histopathological studies were performed on the cartilage of the knee joints. Immunohistochemical analysis was performed to examine the effect of calcitonin on transforming growth factor (TGF)-β1 expression in articular cartilage chondrocytes. Rats subjected to ACLT + OVX surgery showed obvious OA changes in the joints. Animals subjected to ACLT + OVX and treated with calcitonin showed significantly less cartilage degeneration and improved nociceptive tests compared with animals subjected to ACLT + OVX surgeries alone. Moreover, calcitonin increased TGF-β1 expression in chondrocytes in ACLT + OVX-affected cartilage. Subcutaneous injection of calcitonin (1) attenuated the development of OA, (2) concomitantly reduced nociception, and (3) modulated chondrocyte metabolism, possibly by increasing cellular TGF-β1 expression.

摘要

我们研究了降钙素（密盖息）在骨关节炎（OA）进展中的作用，以及在 OA 和骨质疏松症的实验性大鼠模型中的伤害感受行为。OA 通过右膝关节前交叉韧带切断术（ACLT）和双侧卵巢切除术（OVX）诱导 Wistar 大鼠。在手术前和手术后 12 周开始，每周分析伤害感受行为（继发性机械性感觉过敏和后爪负重分布），直到 20 周。在 20 周时，对膝关节软骨进行组织病理学研究。进行免疫组织化学分析，以检查降钙素对关节软骨细胞中转化生长因子（TGF）-β1 表达的影响。接受 ACLT + OVX 手术的大鼠关节明显发生 OA 变化。与仅接受 ACLT + OVX 手术的动物相比，接受 ACLT + OVX 和降钙素治疗的动物的软骨退变明显减少，伤害感受测试得到改善。此外，降钙素增加了 ACLT + OVX 影响软骨中软骨细胞的 TGF-β1 表达。降钙素皮下注射（1）减轻 OA 的发展，（2）同时减轻疼痛，（3）调节软骨细胞代谢，可能通过增加细胞 TGF-β1 表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8aae/4921823/e3cf0783d371/srep28862-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8aae/4921823/e0dc0b754993/srep28862-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8aae/4921823/0424c881c1ea/srep28862-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8aae/4921823/6045fd9d8784/srep28862-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8aae/4921823/c785dbc1a2b9/srep28862-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8aae/4921823/e3cf0783d371/srep28862-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8aae/4921823/e0dc0b754993/srep28862-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8aae/4921823/0424c881c1ea/srep28862-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8aae/4921823/6045fd9d8784/srep28862-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8aae/4921823/c785dbc1a2b9/srep28862-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8aae/4921823/e3cf0783d371/srep28862-f5.jpg

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