从脊髓性肌萎缩症患者中获取人类胚胎干细胞。
Derivation of human embryonic stem cell from spinal muscular atrophy patient.
作者信息
Xie Pingyuan, Zhou Hao, Zhou Xiaoying, Zhao Xiaomeng, Du Juan, Lu Guangxiu, Lin Ge, Ouyang Qi
机构信息
Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China.
Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
出版信息
Stem Cell Res. 2016 Mar;16(2):216-9. doi: 10.1016/j.scr.2015.12.030. Epub 2016 Jan 3.
We established a human embryonic stem cell (hESC) line chHES-427 from the abnormal embryo carrying homozygous deletion of exon 7 of survival motor neuron gene (SMN). This cell line maintained a normal karyotype 46, XX during long-term culture. Further characteristic analysis suggested that the cells expressed the pluripotency-related markers and had the capacity to differentiate into the derivatives from the three germ layers in vitro.
我们从携带生存运动神经元基因（SMN）第7外显子纯合缺失的异常胚胎中建立了人胚胎干细胞（hESC）系chHES-427。该细胞系在长期培养过程中保持了正常的核型46, XX。进一步的特性分析表明，这些细胞表达多能性相关标志物，并且在体外具有分化为三个胚层衍生物的能力。
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