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携带SMN1基因突变的KCL026研究级人胚胎干细胞系的产生。

Generation of KCL026 research grade human embryonic stem cell line carrying a mutation in SMN1 gene.

作者信息

Hewitson Heema, Wood Victoria, Kadeva Neli, Cornwell Glenda, Codognotto Stefano, Stephenson Emma, Ilic Dusko

机构信息

Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.

Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.

出版信息

Stem Cell Res. 2016 Mar;16(2):249-51. doi: 10.1016/j.scr.2016.01.005. Epub 2016 Jan 14.

DOI:10.1016/j.scr.2016.01.005
PMID:27345977
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4823667/
Abstract

The KCL026 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the SMN1 gene encoding survival of motor neuron 1, telomeric (exons 7 and 8 deletion). Mutations in this gene are associated with spinal muscular atrophy. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

摘要

KCL026人胚胎干细胞系源自一个捐赠用于研究的胚胎，该胚胎携带编码运动神经元存活蛋白1的SMN1基因的突变（端粒区，外显子7和8缺失）。该基因的突变与脊髓性肌萎缩症相关。使用激光显微手术分离内细胞团，并接种于经γ射线照射的人包皮成纤维细胞上。细胞系的建立和传代培养均在无动物源产品的环境中进行。通过体外试验确认了其多能状态和分化潜能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9577/4823667/8e22a0839cfc/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9577/4823667/802d9a237aee/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9577/4823667/f55258733136/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9577/4823667/8e22a0839cfc/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9577/4823667/802d9a237aee/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9577/4823667/f55258733136/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9577/4823667/8e22a0839cfc/gr3.jpg

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本文引用的文献

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3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells.人胚胎干细胞和诱导多能干细胞构建的功能性表皮渗透屏障的 3D 体外模型。
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无饲养层且无异源条件下人类胚胎干细胞的分离和扩增。
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