Lau Ho-Tak, Liu Lizhi, Li Xiajun
Department of Developmental and Regenerative Biology, Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY 10029, USA.
Department of Developmental and Regenerative Biology, Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY 10029, USA; Department of Oncological Sciences, Graduate School of Biological Sciences, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY 10029, USA.
Stem Cell Res. 2016 Mar;16(2):282-6. doi: 10.1016/j.scr.2015.12.038. Epub 2016 Jan 6.
Zfp57 is a master regulator of genomic imprinting in mouse embryos. To further test its functions, we have derived multiple Zfp57 mutant ES clones directly from mouse blastocysts. Indeed, we found DNA methylation imprint was lost at most examined imprinting control regions in these Zfp57 mutant ES clones, similar to what was observed in Zfp57 mutant embryos in the previous studies. This result indicates that these blastocyst-derived Zfp57 mutant ES clones can be employed for functional analyses of Zfp57 in genomic imprinting.
Zfp57是小鼠胚胎基因组印记的主要调控因子。为了进一步测试其功能，我们直接从小鼠囊胚中获得了多个Zfp57突变体胚胎干细胞（ES）克隆。实际上，我们发现这些Zfp57突变体ES克隆中，在大多数检测的印记控制区域，DNA甲基化印记丢失，这与之前研究中在Zfp57突变体胚胎中观察到的情况相似。这一结果表明，这些源自囊胚的Zfp57突变体ES克隆可用于Zfp57在基因组印记中的功能分析。
