Dumevska Biljana, Schaft Julia, McKernan Robert, Hu Jesselyn, Schmidt Uli
Genea Biocells, Sydney, Australia.
Genea Biocells, Sydney, Australia.
Stem Cell Res. 2016 Mar;16(2):449-51. doi: 10.1016/j.scr.2016.02.022. Epub 2016 Feb 11.
The Genea091 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying Htt gene CAG expansion of 40 repeats, indicative of Huntington Disease. Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XX by CGH and STR analysis demonstrated a female Allele pattern. The hESC line had pluripotent cell morphology, 92% of cells expressed Nanog, 97% Oct4, 79% Tra1-60 and 98% SSEA4 and gave a Pluritest pluripotency score of 38.36, Novelty of 1.35. The cell line was negative for Mycoplasma and visible contamination.
Genea091人胚胎干细胞系源自一个捐赠的、已获得完全商业同意的辅助生殖技术囊胚，该囊胚携带亨廷顿病相关的Htt基因CAG重复扩增40次。在灭活的人饲养层上培养内细胞团长出后，通过比较基因组杂交（CGH）确认核型为46, XX，短串联重复序列（STR）分析显示为女性等位基因模式。该人胚胎干细胞系具有多能干细胞形态，92%的细胞表达Nanog，97%表达Oct4，79%表达Tra1-60，98%表达阶段特异性胚胎抗原4（SSEA4），多能性检测Pluritest评分为38.36，新颖性评分为1.35。该细胞系支原体检测为阴性，且无可见污染。
