Gao Liyang, Zhao Mingyan, Ye Wei, Huang Jinzhi, Chu Jiaqi, Yan Shouquan, Wang Chaojun, Zeng Rong
Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
Tissue Cell. 2016 Aug;48(4):312-20. doi: 10.1016/j.tice.2016.06.001. Epub 2016 Jun 3.
The amniotic fluid has a heterogeneous population of cells. Some human amniotic fluid-derived stem (hAFS) cells have been shown to harbor the potential to differentiate into neural cells. However, the neural differentiation efficiency of hAFS cells remains low. In this study, we isolated CD117-positive hAFS cells from amniotic fluid and then examined the pluripotency of these cells through the formation of embryoid bodies (EBs). Additionally, we induced the neural differentiation of these cells using neuroectodermal medium. This study revealed that the GSK3-beta inhibitor SB216763 was able to stimulate the proliferation of CD117-positive hAFS cells without influencing their undifferentiated state. Moreover, SB216763 can efficiently promote the neural differentiation of CD117-positive hAFS cells towards neural progenitor cells in the presence of DMEM/F12 and N2 supplement. These findings provide an easy and low-cost method to maintain the proliferation of hAFS cells, as well as induce an efficacious generation of neural progenitor cells from hAFS cells. Such induction of the neural commitment of hAFS cells may provide an option for the treatment of neurodegenerative diseases by hAFS cells-based therapies.
羊水具有异质性细胞群体。一些人羊水来源的干细胞（hAFS细胞）已被证明具有分化为神经细胞的潜力。然而，hAFS细胞的神经分化效率仍然较低。在本研究中，我们从羊水中分离出CD117阳性的hAFS细胞，然后通过形成胚状体（EBs）来检测这些细胞的多能性。此外，我们使用神经外胚层培养基诱导这些细胞进行神经分化。本研究表明，GSK3-β抑制剂SB216763能够刺激CD117阳性hAFS细胞的增殖，而不影响其未分化状态。此外，在存在DMEM/F12和N2补充剂的情况下，SB216763可以有效地促进CD117阳性hAFS细胞向神经祖细胞的神经分化。这些发现提供了一种简单且低成本的方法来维持hAFS细胞的增殖，以及从hAFS细胞诱导产生有效的神经祖细胞。hAFS细胞这种神经定向诱导可能为基于hAFS细胞的疗法治疗神经退行性疾病提供一种选择。
