硝普钠在大鼠离体、灌注、无血肝脏和后肢中的氰化物释放。

Cyanide release from nitroprusside in the isolated, perfused, bloodless liver and hindlimbs of the rat.

作者信息

Devlin D J, Smith R P, Thron C D

机构信息

Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, New Hampshire 03756.

出版信息

Toxicol Appl Pharmacol. 1989 Jun 15;99(2):354-6. doi: 10.1016/0041-008x(89)90017-3.

DOI:10.1016/0041-008x(89)90017-3

PMID:2734796

Abstract

When sodium nitroprusside in artificial medium was perfused through the isolated liver and hindlimbs of a rat at the near physiological flow rate of 8.5 ml min-1, free cyanide was found in the perfusate. The liver reached a steady-state ratio of cyanide released/nitroprusside perfused of about 1.5 (or approximately 30% of the total nitroprusside cyanide) within 15 min, and maintained that rate for about 1.5 hr. In the hindlimbs cyanide was released at a much slower rate (7.5 to 18.8% of the total), and the release did not achieve a steady state even after 1.5 hr. Even after small corrections for cyanide extraction by both tissues, the rate of cyanide release by either tissue was probably more rapid than that resulting from static incubations in blood.

摘要

当以接近生理流速8.5毫升/分钟的速度，将人工培养基中的硝普钠灌注到大鼠离体肝脏和后肢时，在灌注液中发现了游离氰化物。肝脏在15分钟内达到了氰化物释放量/灌注硝普钠量的稳态比率，约为1.5（或约占总硝普钠氰化物的30%），并在约1.5小时内维持该比率。在后肢中，氰化物的释放速度要慢得多（占总量的7.5%至18.8%），即使在1.5小时后释放也未达到稳态。即使对两个组织提取氰化物的情况进行了小的校正，任一组织的氰化物释放速度可能都比在血液中静态孵育产生的速度更快。