Norris J C, Hume A S
Br J Anaesth. 1987 Feb;59(2):236-9. doi: 10.1093/bja/59.2.236.
Brain cytochrome oxidase activity was measured after the in vitro addition of potassium cyanide (KCN) or sodium nitroprusside (SNP). Activity of cytochrome oxidase was sensitive to KCN; however, this activity was unaffected by SNP. In SNP- and KCN-treated animals brain cytochrome oxidase activities were measured. At 3 min after SNP injection, inhibition of the enzymatic activity was the same as 1 min after KCN injection. Time to death for SNP-treated animals was longer than for KCN-treated animals. These data suggest that cyanide was released in vivo from SNP and that time was necessary for this release to occur.
在体外添加氰化钾（KCN）或硝普钠（SNP）后，测量脑细胞色素氧化酶活性。细胞色素氧化酶活性对KCN敏感；然而，该活性不受SNP影响。在经SNP和KCN处理的动物中测量脑细胞色素氧化酶活性。注射SNP后3分钟，酶活性的抑制程度与注射KCN后1分钟相同。SNP处理动物的死亡时间比KCN处理动物长。这些数据表明，体内SNP释放出了氰化物，且这种释放需要时间。
