Chen Xiaojuan, Zhang Jianguo, Wang Xianjun, Bi Jianzhong
Departments of aNeurology bCritical Care Medicine, Linyi People's Hospital Affiliated to Shandong University, Linyi cDepartment of Neurology, the Second Hospital Affiliated to Shandong University, Jinan, China.
Neuroreport. 2016 Aug 17;27(12):940-7. doi: 10.1097/WNR.0000000000000635.
Peripheral nerve injuries are becoming more common, but without effective treatment, the outcome is often very poor. Recent research shows that p75 plays an important role in nerve regeneration, but its mechanisms of action during behavioral recovery and axon regrowth remain unclear. To investigate these mechanisms, we examined recovery from sciatic nerve crush injury in wild-type and p75 knockout mice. We found that sciatic nerve crush injury upregulates mRNA and protein expressions of p75 and p75 deficiency alters gene and protein expression of molecules associated with distal portion atrophy. However, p75 deletion did not alter gene and protein expression in the spinal cord of molecules related to neuronal intrinsic growth capacity. Behavioral testing showed that functional recovery was delayed in mice lacking p75. These results suggest that p75 regulates gene and protein expression that limits the distal atrophy after sciatic nerve injury, thereby regulating axonal growth and functional recovery.
周围神经损伤正变得越来越常见，但如果没有有效的治疗方法，结果往往非常糟糕。最近的研究表明，p75在神经再生中起重要作用，但其在行为恢复和轴突再生过程中的作用机制仍不清楚。为了研究这些机制，我们检测了野生型和p75基因敲除小鼠坐骨神经挤压伤后的恢复情况。我们发现，坐骨神经挤压伤会上调p75的mRNA和蛋白表达，而p75缺乏会改变与远端萎缩相关分子的基因和蛋白表达。然而，p75缺失并没有改变脊髓中与神经元内在生长能力相关分子的基因和蛋白表达。行为测试表明，缺乏p75的小鼠功能恢复延迟。这些结果表明，p75调节基因和蛋白表达，限制坐骨神经损伤后的远端萎缩，从而调节轴突生长和功能恢复。
