Segeletz Sandra, Hoflack Bernard
Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
Proteomics. 2016 Oct;16(19):2545-2556. doi: 10.1002/pmic.201500519. Epub 2016 Aug 4.
Bone is a dynamic tissue whose remodeling throughout life is orchestrated by repeated cycles of destruction mediated by osteoclasts and rebuilding by osteoblasts. Current understanding of osteoclast biology has largely relied on the generation of knockout mice exhibiting an abnormal bone phenotype. This has provided a better understanding of osteoclast biology and the key proteins that support osteoclast function. However, mouse models alone do not provide an integrated view on protein networks and post-translational modifications that might be important for osteoclast function. During the past years, a number of MS-based quantitative methods have been developed to investigate the complexity of biological systems. This review will summarize how such approaches have contributed to the understanding of osteoclast differentiation and function.
骨骼是一种动态组织，其在整个生命过程中的重塑由破骨细胞介导的反复破坏周期和成骨细胞的重建精心安排。目前对破骨细胞生物学的理解很大程度上依赖于表现出异常骨表型的基因敲除小鼠的产生。这为破骨细胞生物学以及支持破骨细胞功能的关键蛋白提供了更好的理解。然而，仅靠小鼠模型并不能提供关于可能对破骨细胞功能很重要的蛋白质网络和翻译后修饰的综合观点。在过去几年中，已经开发了许多基于质谱的定量方法来研究生物系统的复杂性。本综述将总结这些方法如何有助于对破骨细胞分化和功能的理解。
