Zhang Qian, Lyu Shuxia
College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning, China.
Front Microbiol. 2023 Mar 21;14:1131000. doi: 10.3389/fmicb.2023.1131000. eCollection 2023.
Microbial consortia play a key role in human health, bioenergy, and food manufacturing due to their strong stability, robustness and versatility. One of the microbial consortia consisting of and for the production of the vitamin C precursor, 2-keto-L-gulonic acid (2-KLG), has been widely used for large-scale industrial production. To further investigate the cell-cell communication in microbial consortia, a microbial consortium consisting of and was constructed and the differences in protein expression at different fermentation time points (18 h and 40 h) were analyzed by iTRAQ-based proteomics. The results indicated that was subjected to acid shocks in the coculture fermentation system and responded to it. In addition, the quorum sensing system existed in the coculture fermentation system, and could secrete quorum-quenching lactonase (YtnP) to inhibit the signaling pathway of . This study offers valuable guidance for further studies of synthetic microbial consortia.
微生物群落由于其强大的稳定性、鲁棒性和多功能性，在人类健康、生物能源和食品制造中发挥着关键作用。其中一种由[具体菌种1]和[具体菌种2]组成的用于生产维生素C前体2-酮基-L-古龙酸（2-KLG）的微生物群落已被广泛用于大规模工业生产。为了进一步研究微生物群落中的细胞间通讯，构建了一个由[具体菌种1]和[具体菌种2]组成的微生物群落，并通过基于iTRAQ的蛋白质组学分析了不同发酵时间点（18小时和40小时）的蛋白质表达差异。结果表明，[具体菌种1]在共培养发酵系统中受到酸冲击并做出了响应。此外，共培养发酵系统中存在群体感应系统，[具体菌种2]可以分泌群体淬灭内酯酶（YtnP）来抑制[具体菌种1]的信号通路。本研究为合成微生物群落的进一步研究提供了有价值的指导。
