Kyoui Daisuke, Hirokawa Eri, Takahashi Hajime, Kuda Takashi, Kimura Bon
a Department of Food Science and Technology, Faculty of Marine Science , Tokyo University of Marine Science and Technology , Tokyo , Japan.
Biofouling. 2016 Aug;32(7):815-26. doi: 10.1080/08927014.2016.1198953.
Listeria monocytogenes is an important cause of human foodborne infections and its ability to form biofilms is a serious concern to the food industry. To reveal the effect of glucose conditions on biofilm formation of L. monocytogenes, 20 strains were investigated under three glucose conditions (0.1, 1.0, and 2.0% w v(-1)) by quantifying the number of cells in the biofilm and observing the biofilm structure after incubation for 24, 72, and 168 h. In addition, the biofilms were examined for their sensitivity to sodium hypochlorite. It was found that high concentrations of glucose reduced the number of viable cells in the biofilms and increased extracellular polymeric substance production. Moreover, biofilms formed at a glucose concentration of 1.0 or 2.0% were more resistant to sodium hypochlorite than those formed at a glucose concentration of 0.1%. This knowledge can be used to help design the most appropriate sanitation strategy.
单核细胞增生李斯特菌是人类食源性感染的一个重要病因，其形成生物膜的能力是食品行业严重关切的问题。为揭示葡萄糖条件对单核细胞增生李斯特菌生物膜形成的影响，在三种葡萄糖条件（0.1%、1.0%和2.0% w v⁻¹）下，通过对生物膜中的细胞数量进行定量并观察培养24、72和168小时后的生物膜结构，对20株菌株进行了研究。此外，还检测了生物膜对次氯酸钠的敏感性。结果发现，高浓度葡萄糖减少了生物膜中活细胞的数量，并增加了细胞外聚合物的产生。此外，葡萄糖浓度为1.0%或2.0%时形成的生物膜比葡萄糖浓度为0.1%时形成的生物膜对次氯酸钠更具抗性。这些知识可用于帮助设计最合适的卫生策略。
