Alzahrani Yahya, Colorado Luisa H, Pritchard Nicola, Efron Nathan
Institute of Health and Biomedical Innovation, and School of Optometry and Vision Science, Queensland University of Technology, Kelvin Grove, Queensland, Australia.
Clin Exp Optom. 2017 Jan;100(1):33-40. doi: 10.1111/cxo.12399. Epub 2016 Jun 28.
The aim was to determine longitudinal changes in Langerhans cell density (LCD) in the human cornea and conjunctiva during asymptomatic and symptomatic contact lens wear.
Twenty-five participants with contact lens-induced dry eye (CLIDE) and 35 without CLIDE (NO-CLIDE), diagnosed using a range of symptom questionnaires and objective tests (tear film break up, cotton thread tear test and corneal staining) were enrolled. The central cornea and nasal bulbar conjunctiva were examined using a Heidelberg laser scanning confocal microscope at baseline and following one, four and 24 weeks wear of daily disposable hydrogel contact lenses. Twenty-three non-contact lens-wearing controls were also examined. Langerhans cells were counted manually from randomly selected images.
In the cornea, mean and standard error of the mean LCD was greater after one week of lens wear in CLIDE (55 ± 7 cells/mm ) versus NO-CLIDE (43 ± 4 cells/mm ) (p = 0.041) and controls (27 ± 4 cells/mm ) (p < 0.001). LCD was also greater in NO-CLIDE versus controls (p = 0.010). At week 4, LCD was greater in CLIDE (41 ± 6 cells/mm ) versus controls (27 ± 4 cells/mm ) (p = 0.004). There were no other significant differences between groups at weeks four or 24. In the conjunctiva, LCD was greater after one week of lens wear in CLIDE (17 ± 1 cells/mm ) (p = 0.003) and NO-CLIDE (17 ± 3 cells/mm ) (p = 0.001) versus controls (7 ± 1 cells/mm ). There were no significant differences between groups at weeks four or 24.
The initial transient increase in corneal and conjunctival LCD in CLIDE (versus NO-CLIDE) suggests an inflammatory component in the aetiology of this condition.
目的是确定在无症状和有症状的隐形眼镜佩戴期间，人角膜和结膜中朗格汉斯细胞密度（LCD）的纵向变化。
招募了25名患有隐形眼镜诱发的干眼（CLIDE）的参与者和35名无CLIDE（NO-CLIDE）的参与者，使用一系列症状问卷和客观测试（泪膜破裂、棉线泪液试验和角膜染色）进行诊断。在基线以及每日佩戴一次性水凝胶隐形眼镜1周、4周和24周后，使用海德堡激光扫描共聚焦显微镜检查中央角膜和鼻侧球结膜。还检查了23名不佩戴隐形眼镜的对照者。从随机选择的图像中手动计数朗格汉斯细胞。
在角膜中，CLIDE组佩戴镜片1周后的平均LCD及其平均标准误（55±7个细胞/mm）高于NO-CLIDE组（43±4个细胞/mm）（p = 0.041）和对照组（27±4个细胞/mm）（p < 0.001）。NO-CLIDE组的LCD也高于对照组（p = 0.010）。在第4周时，CLIDE组的LCD（41±6个细胞/mm）高于对照组（27±4个细胞/mm）（p = 0.004）。在第4周或24周时，各组之间没有其他显著差异。在结膜中，CLIDE组（17±1个细胞/mm）（p = 0.003）和NO-CLIDE组（17±3个细胞/mm）（p = 0.001）佩戴镜片1周后的LCD高于对照组（7±1个细胞/mm）。在第4周或24周时，各组之间没有显著差异。
CLIDE组（相对于NO-CLIDE组）角膜和结膜LCD最初的短暂增加表明该病症的病因中存在炎症成分。
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