使用结合硬脂酸修饰的八聚精氨酸的阳离子脂质体递送小干扰RNA。

Delivery of siRNA Using Cationic Liposomes Incorporating Stearic Acid-modified Octa-Arginine.

作者信息

Yang Dongsheng, Li Yuhuan, Qi Yuhang, Chen Yongzhen, Yang Xuewei, Li Yujing, Liu Songcai, Lee Robert J

机构信息

College of Animal Science, Jilin University, Changchun, Jilin, P.R. China Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, Guangdong, P.R. China.

College of Life Sciences, Jilin University, Changchun, Jilin, P.R. China.

出版信息

Anticancer Res. 2016 Jul;36(7):3271-6.

PMID:27354583

Abstract

Cationic liposomes incorporating stearic acid-modified octa-arginine (StA-R8) were evaluated for survivin small interfering RNA (siRNA) delivery. StA-R8 was synthesized and incorporated into liposomes. The composition of liposomes was optimized. Physicochemical properties, cytotoxicity, cellular uptake and gene silencing activity of the liposomes complexed to survivin siRNA were investigated. The results showed that StA-R8-containing liposomes had reduced cytotoxicity and improved delivery efficiency of siRNA into cancer cells compared with StA-R8 by itself.

摘要

评估了包含硬脂酸修饰的八聚精氨酸（StA-R8）的阳离子脂质体用于生存素小干扰RNA（siRNA）递送的效果。合成了StA-R8并将其掺入脂质体中。对脂质体的组成进行了优化。研究了与生存素siRNA复合的脂质体的物理化学性质、细胞毒性、细胞摄取和基因沉默活性。结果表明，与单独的StA-R8相比，含StA-R8的脂质体具有降低的细胞毒性，并提高了siRNA向癌细胞的递送效率。