Li Feng, Jiang Tao, Liu Wei, Hu Quan, Yin Huinan
Department of Burns and Plastic Surgery, The First Affiliated Hospital of the General Hospital of People's Liberation Army of China, Beijing 100048, P.R. China.
Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, P.R. China.
Mol Med Rep. 2016 Aug;14(2):1667-72. doi: 10.3892/mmr.2016.5442. Epub 2016 Jun 23.
Hyperglycemia is the key clinical feature of diabetes, and may induce refractory wound lesions and impaired angiogenesis. Dracorhodin perchlorate (Dra) is the major ingredient of dragon's blood and it has been used as a medicine to treat chronic wounds, such as diabetic foot, since ancient times in many cultures. The current study aimed to investigate the effect of Dra on human umbilical vein endothelial cells (HUVECs) under high‑glucose (HG) stimulation and its potential mechanism. Dra was observed to increase the multiplication capacity of HUVECs both under low glucose (LG) and HG concentrations. Additionally, migration and tube formation in HUVECs was facilitated by Dra. The expression levels of Ras, mitogen‑activated protein kinase (MAPK) and vascular endothelial growth factor, which are key components of the Ras/MAPK pathway, were upregulated following Dra treatment. The present study is the first report, to the best of our knowledge, of the effects of Dra on wound healing, and the association with the Ras/MAPK signaling pathway.
高血糖是糖尿病的关键临床特征，可能导致难治性伤口病变和血管生成受损。高氯酸血竭素（Dra）是血竭的主要成分，自古以来在许多文化中都被用作治疗慢性伤口（如糖尿病足）的药物。本研究旨在探讨Dra在高糖（HG）刺激下对人脐静脉内皮细胞（HUVECs）的影响及其潜在机制。观察到Dra在低葡萄糖（LG）和HG浓度下均能增加HUVECs的增殖能力。此外，Dra促进了HUVECs的迁移和管腔形成。Dra处理后，Ras、丝裂原活化蛋白激酶（MAPK）和血管内皮生长因子（Ras/MAPK途径的关键成分）的表达水平上调。据我们所知，本研究是Dra对伤口愈合影响及其与Ras/MAPK信号通路关联的首次报道。
