Ojo Babajide, El-Rassi Guadalupe Davila, Payton Mark E, Perkins-Veazie Penelope, Clarke Stephen, Smith Brenda J, Lucas Edralin A
Department of Nutritional Sciences.
Food and Agricultural Products Center, and.
J Nutr. 2016 Aug;146(8):1483-91. doi: 10.3945/jn.115.226688. Epub 2016 Jun 29.
High-fat (HF) diet-induced obesity is associated with changes in the gut microbiota. Fiber and other bioactive compounds in plant-based foods are suggested to prevent gut dysbiosis brought on by HF feeding. Mango is high in fiber and has been reported to have anti-obesogenic, hypoglycemic, and immunomodulatory properties.
We investigated the effects of freeze-dried mango pulp combined with an HF diet on the cecal microbial population and its relation to body composition, lipids, glucose parameters, short-chain fatty acid (SCFA) production, and gut inflammatory markers in a mouse model of diet-induced obesity.
Six-wk-old male C57BL/6 mice were randomly assigned to 1 of 4 dietary treatment groups: control (AIN-93M, 10% fat kcal), HF (60% fat kcal), and HF + 1% or 10% mango (HF+1%M or HF+10%M, wt:wt) for 12 wk. The cecal microbial population was assessed by use of 16S rDNA sequencing. Body composition, plasma glucose and lipids, cecal and fecal SCFAs, and mRNA abundance of inflammatory markers in the ileum and colonic lamina propria were assessed.
Compared with the control group, HF feeding significantly reduced (P < 0.05) 1 operational taxonomic unit (OTU) of the genus Bifidobacteria (64-fold) and 5 OTUs of the genus Akkermansia (≥16-fold). This reduction was prevented in the HF+10%M group, members of which had 10% higher final body weight compared with the HF group (P = 0.01) and similar fasting blood glucose concentrations (P = 0.24). The HF+10%M group had 135% (P = 0.004) and 133% (P < 0.0001) greater fecal acetic and n-butyric acids concentrations than the HF group, suggesting greater microbial fermentation. Furthermore, a 59% greater colonic interleukin 10 (Il10) gene expression was observed in the HF+10%M group than in the HF group (P = 0.048), indicating modulation of gut inflammation. The HF+1%M group generally did not differ from the HF group.
The addition of mango to an HF diet modulated the gut microbiota and production of SCFAs in C57BL/6 mice; these changes may improve gut tolerance to the insult of an HF diet.
高脂（HF）饮食诱导的肥胖与肠道微生物群的变化有关。植物性食物中的纤维和其他生物活性化合物被认为可以预防高脂喂养引起的肠道菌群失调。芒果富含纤维，据报道具有抗肥胖、降血糖和免疫调节特性。
我们在饮食诱导肥胖的小鼠模型中研究了冻干芒果果肉与高脂饮食联合使用对盲肠微生物群的影响及其与身体组成、脂质、血糖参数、短链脂肪酸（SCFA）产生和肠道炎症标志物的关系。
将6周龄雄性C57BL/6小鼠随机分为4个饮食处理组中的1组：对照组（AIN-93M，10%脂肪千卡）、高脂组（60%脂肪千卡）以及高脂+1%或10%芒果组（HF+1%M或HF+10%M，重量比），持续12周。通过16S rDNA测序评估盲肠微生物群。评估身体组成、血浆葡萄糖和脂质、盲肠和粪便中的SCFA以及回肠和结肠固有层中炎症标志物的mRNA丰度。
与对照组相比，高脂喂养显著降低（P<0.05）了双歧杆菌属的1个操作分类单元（OTU）（64倍）和阿克曼氏菌属的5个OTU（≥16倍）。在HF+10%M组中这种降低得到了预防，该组小鼠的终末体重比高脂组高10%（P=0.01），且空腹血糖浓度相似（P=0.24）。HF+10%M组的粪便乙酸和正丁酸浓度比高脂组分别高135%（P=0.004）和133%（P<0.0001），表明微生物发酵更强。此外，与高脂组相比，HF+10%M组的结肠白细胞介素10（Il10）基因表达高59%（P=0.048），表明肠道炎症得到调节。HF+1%M组与高脂组一般无差异。
在高脂饮食中添加芒果可调节C57BL/6小鼠的肠道微生物群和SCFA的产生；这些变化可能会提高肠道对高脂饮食损害的耐受性。
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