来自卢旺达农村地区儿童粪便样本中的钩虫样虫卵。
Hookworm-like eggs in children's faecal samples from a rural area of Rwanda.
作者信息
Irisarri-Gutiérrez María José, Muñoz-Antolí Carla, Acosta Lucrecia, Parker Lucy Anne, Toledo Rafael, Bornay-Llinares Fernando Jorge, Esteban José Guillermo
机构信息
Área de Parasitología del Departament de Biología Cel.lular i Parasitología, Facultat de Farmàcia, Universitat de València, Valencia, Spain.
Área de Parasitología del Departamento de Agroquímica y Medioambiente, Facultad de Farmacia, Universidad Miguel Hernández de Elche, Alicante, Spain; Laboratorio de Análisis Clínicos, Sanatorio "Fontilles", Vall de Laguar, Alicante, Spain.
出版信息
Afr Health Sci. 2016 Mar;16(1):83-8. doi: 10.4314/ahs.v16i1.11.
BACKGROUND
Hookworm eggs identification and quantification is usually carried out by Kato-Katz method. However various structures present in the smear may be confused with eggs of such parasites.
OBJECTIVE
To document the presence of structures in Kato-Katz slides that could initially be misinterpreted as hookworm eggs.
METHOD
497 faecal samples were analysed by Kato-Katz technique, diphasic concentration technique, agar-plate coprocultive and larvae obtained were analysed by PCR and characterized by sequencing.
RESULT
Hookworm-like eggs were found in 159 (32%) of the samples by Kato-Katz, finally identified as Caenorhabditis elegans by PCR technique.
CONCLUSION
The diagnosis of human hookworm eggs, only by the use of Kato-Katz technique can lead to false positives because of similarities with eggs of other free-living worms, from wet soils like those of Rwanda that could contaminate stool samples.
背景
钩虫卵的鉴定和定量通常采用改良加藤厚涂片法。然而，涂片中存在的各种结构可能会与这类寄生虫的卵混淆。
目的
记录改良加藤厚涂片载玻片上最初可能被误判为钩虫卵的结构。
方法
采用改良加藤厚涂片技术、双相浓缩技术、琼脂平板粪便培养法对497份粪便样本进行分析，并对获得的幼虫进行聚合酶链反应（PCR）分析及测序鉴定。
结果
改良加藤厚涂片法在159份（32%）样本中发现了类似钩虫的卵，最终通过PCR技术鉴定为秀丽隐杆线虫。
结论
仅使用改良加藤厚涂片技术诊断人体钩虫卵可能会出现假阳性结果，因为卢旺达潮湿土壤中的其他自由生活蠕虫的卵与钩虫卵相似，可能会污染粪便样本。
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