Barrière Antoine, Félix Marie-Anne
Institut de Biologie du Développement de Marseille-Luminy, CNRS, France.
WormBook. 2014 May 2:1-19. doi: 10.1895/wormbook.1.115.2.
Isolating Caenorhabditis and other nematodes from the wild first requires field sampling (reviewed in Section 1). The easiest and most efficient way to recover the animals from any substrate is to place the sample onto a standard C. elegans culture plate (Section 2.1). Alternative methods used by nematologists to recover soil nematodes (Sections 2.2, 2.3, and 2.4) are in our hands more difficult to implement and only yield a fraction of the individuals in the sample. A tricky step is to recognize your species of interest out of the zoo of nematode species that comes with a typical sample (Section 3). Culture (Section 4) and freezing (Section 5) conditions are then reviewed. Finally, we briefly summarize the organization and timing of an isolation experiment (Section 6), as well as the available collections (Section 7). Bear in mind that this chapter is strongly focused towards the isolation of Caenorhabditis elegans and close relatives.
首先，从野外分离秀丽隐杆线虫和其他线虫需要进行野外采样（第1节有相关综述）。从任何基质中获取线虫的最简单、最有效的方法是将样品置于标准的秀丽隐杆线虫培养板上（第2.1节）。线虫学家用于获取土壤线虫的其他方法（第2.2、2.3和2.4节），在我们看来实施起来更困难，而且只能获取样品中一小部分个体。一个棘手的步骤是从典型样品中形形色色的线虫种类中识别出你感兴趣的物种（第3节）。接下来会探讨培养条件（第4节）和冷冻条件（第5节）。最后，我们简要总结分离实验的流程和时间安排（第6节）以及现有的线虫样本库（第7节）。请记住，本章重点聚焦于秀丽隐杆线虫及其近缘种的分离。
