Division of Allergy and Immunology, Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Department of Otorhinolaryngology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Asian Pac J Allergy Immunol. 2016 Dec;34(4):295-299. doi: 10.12932/AP0737.
The skin prick test (SPT) is a valid and approved tool that is used to diagnose atopic diseases. The SPT is accurate, safe, simple and inexpensive. However, the histamine concentration used as a positive control in the SPT varies among centers.
To compare SPT results using different concentrations of locally-prepared and imported histamine solutions.
This prospective, randomized, double-blind, self-controlled study was performed in healthy adult volunteers. The SPT was performed using 4 concentrations of histamine solutions (imported, 1 mg/mL; locally-prepared, 1, 5 and 10 mg/mL). Locally-prepared histamine positive controls were prepared from histamine biphosphate monohydrate using sterile technique.
Seventy-five adult volunteers (mean age, 36 years) were included in the study. Eight volunteers were male and 9 had a history of atopy. Mean wheal diameter (MWD) for imported histamine was 3.49 mm for a concentration of 1 mg/mL, and that of locally-prepared histamine was 2.94 mm, 5.05 mm and 5.52 mm for concentrations of 1, 5 and 10 mg/mL histamine, respectively. Negative SPT results (MWD <3 mm) were found in 11 subjects (14.7%) who received imported histamine and 26 subjects (34.7%) who received the locally-prepared histamine at concentration of 1 mg/mL. All subjects tested with locally-prepared histamine at concentrations of 5 and 10 mg/mL had a MWD > 3 mm.
Locally-prepared histamine base at concentrations of 5 and 10 mg/mL yielded better positive results than both imported and locally-prepared histamine at a concentration of 1 mg/mL.
皮肤点刺试验（SPT）是一种有效的、经过验证的工具，用于诊断特应性疾病。SPT 准确、安全、简单且廉价。然而，SPT 中用作阳性对照的组织胺浓度在各中心之间存在差异。
比较使用不同浓度的本地制备和进口组织胺溶液进行 SPT 的结果。
这是一项前瞻性、随机、双盲、自身对照研究，在健康成年志愿者中进行。使用 4 种浓度的组织胺溶液（进口，1mg/mL；本地制备，1、5 和 10mg/mL）进行 SPT。本地制备的组织胺阳性对照物是使用无菌技术从组织胺二磷酸盐一水合物制备的。
共有 75 名成年志愿者（平均年龄 36 岁）纳入本研究。8 名志愿者为男性，9 名有特应性病史。进口组织胺浓度为 1mg/mL 时的平均风团直径（MWD）为 3.49mm，本地制备的组织胺浓度为 1、5 和 10mg/mL 时的 MWD 分别为 2.94mm、5.05mm 和 5.52mm。接受进口组织胺的 11 名受试者（14.7%）和接受 1mg/mL 浓度的本地制备组织胺的 26 名受试者（34.7%）的 SPT 结果为阴性（MWD<3mm）。所有接受 5 和 10mg/mL 浓度的本地制备组织胺测试的受试者的 MWD 均大于 3mm。
与进口和本地制备的 1mg/mL 浓度的组织胺相比，浓度为 5 和 10mg/mL 的本地制备组织胺碱基产生了更好的阳性结果。
