Hospital Das Clínicas Da Faculdade De Medicina Da Universidade De São Paulo, SP, Brazil.
Centro De Hematologia E Hemoterapia De Santa Catarina - HEMOSC, Brazil.
Cytometry B Clin Cytom. 2017 Sep;92(5):398-410. doi: 10.1002/cyto.b.21396. Epub 2016 Jul 28.
Multiparametric flow cytometry (MFC) is a powerful tool for the diagnosis of hematological malignancies and has been useful for the classification of chronic lymphoproliferative disorders (CLPD) according to the WHO criteria. Following the purposes of the Brazilian Group of Flow Cytometry (GBCFLUX), the aim of this report was to standardize the minimum requirements to achieve an accurate diagnosis in CLPDs, considering the different economic possibilities of the laboratories in our country. Most laboratories in Brazil work with 4-fluorescence flow cytometers, which is why the GBCFLUX CLPD Committee has proposed 4-color monoclonal antibody (MoAb) panels.
METHODS/RESULTS: Panels for screening and diagnosis in B, T and NK lymphoproliferative disorders were developed based on the normal differentiation pathways of these cells and the most frequent phenotypic aberrations. Important markers for prognosis and for minimal residual disease (MRD) evaluation were also included. The MoAb panels presented here were designed based on the diagnostic expertise of the participating laboratories and an extensive literature review.
The 4-color panels presented to aid in the diagnosis of lymphoproliferative neoplasms by GBCFLUX aim to provide clinical laboratories with a systematic, step-wise, cost-effective, and reproducible approach to obtain an accurate immunophenotypic diagnosis of the most frequent of these disorders. © 2016 International Clinical Cytometry Society.
多参数流式细胞术（MFC）是诊断血液系统恶性肿瘤的有力工具，根据世界卫生组织（WHO）标准，对慢性淋巴细胞增殖性疾病（CLPD）的分类也很有用。本报告旨在按照巴西流式细胞术工作组（GBCFLUX）的目的，根据我国实验室的不同经济可能性，标准化 CLPD 准确诊断的最低要求。巴西大多数实验室都使用 4 色流式细胞仪，这就是为什么 GBCFLUX CLPD 委员会提出了 4 色单克隆抗体（MoAb）组合的原因。
方法/结果：基于这些细胞的正常分化途径和最常见的表型异常，制定了用于 B、T 和 NK 淋巴细胞增殖性疾病筛查和诊断的面板。还包括用于预后和微小残留病（MRD）评估的重要标志物。这里提出的 MoAb 面板是根据参与实验室的诊断专业知识和广泛的文献综述设计的。
GBCFLUX 提出的 4 色面板旨在帮助诊断淋巴增殖性肿瘤，旨在为临床实验室提供一种系统、逐步、具有成本效益和可重复的方法，以获得这些疾病中最常见疾病的准确免疫表型诊断。©2016 年国际临床细胞化学学会。
