Eida Omima M, El-Shafei Hanaa A, Nomeir Yousra A, El Safhi Mohammed B
J Egypt Soc Parasitol. 2016 Apr;46(1):27-34. doi: 10.12816/0026147.
Metronidazole (MTZ) was the most widely accepted treatment for Blastocystis hominis (B. hominis) with high treatment failure rate, resistance and potential mutagenic and carcinogenic effects so there is urgent need to find out new, effective and safe treatment against B. hominis. The present research aimed to evaluate the therapeutic effect of the aqueous extract of Nigella sativa (NS) at different doses on B. hominis in vitro and in vivo in comparison to MTZ as a control drug. Isolates of B. hominis were obtained from patients complaining of diarrhea and abdominal pain. Isolates were cultured in egg diphasic medium (LE) for in vitro study and to adjust proper inoculating dose for in vivo study. The aqueous extract of NS at concentrations of 100 & 500 µg/ml showed a potent lethal effect on B. hominis isolates in vitro. Caecal tissue of experimentally infected and treated mice with two different doses of NS (250 & 500 mg/kg/d) were examined histopathologically and compared with that of mice infected and treated by two doses of MTZ (62 & 125 mg/kg/d) as control drug and Infected untreated mice as negative control group. Histopathological examination of infected untreated group showed all pathological degrees in the caecal tissue while infected treated one showed remission of pathological changes especially with higher dose (500 mg/kg). Present study proved that N. sativa had inhibitory effect on B. hominis in vitro and prevented cytopathic effect in infected mice inoculated orally with B. hominis.
甲硝唑（MTZ）是治疗人芽囊原虫（B. hominis）最广泛接受的药物，但治疗失败率高、存在耐药性以及潜在的致突变和致癌作用，因此迫切需要找到针对人芽囊原虫的新的、有效且安全的治疗方法。本研究旨在评估不同剂量的黑种草（NS）水提取物与作为对照药物的MTZ相比，在体外和体内对人芽囊原虫的治疗效果。人芽囊原虫分离株取自抱怨腹泻和腹痛的患者。分离株在卵双相培养基（LE）中培养用于体外研究，并调整适当的接种剂量用于体内研究。浓度为100和500μg/ml的NS水提取物在体外对人芽囊原虫分离株显示出强大的致死作用。对用两种不同剂量的NS（250和500mg/kg/d）进行实验性感染和治疗的小鼠的盲肠组织进行组织病理学检查，并与用两种剂量的MTZ（62和125mg/kg/d）作为对照药物进行感染和治疗的小鼠以及未感染未治疗的小鼠作为阴性对照组进行比较。未感染未治疗组的组织病理学检查显示盲肠组织出现所有病理程度，而感染治疗组显示病理变化有所缓解，尤其是高剂量（500mg/kg）时。本研究证明黑种草在体外对人芽囊原虫有抑制作用，并预防了口服接种人芽囊原虫的感染小鼠的细胞病变效应。
