Arise R O, Tella A C, Akintola A A, Akiode S O, Malomo S O
Department of Biochemistry, University of Ilorin, Kwara State, Nigeria.
Department of Chemistry, University of Ilorin, Kwara State, Nigeria.
EXCLI J. 2012 May 14;11:219-25. eCollection 2012.
The aim of this study was to investigate the effect of crankcase oil on the cellular and functional integrity of rat skin. Thirty (30) rats were randomly grouped into six viz groups A-F. Group A (base-line control) received 2 ml of distilled water. 2.5 %, 5.0 %, 7.5 %, and 10.0 % v/v of the crankcase oil were prepared using unused oil as solvent and 2 ml of the concentrations were topically administered to groups C-F respectively for seven consecutive days. Group B served as positive control and received 2 ml of the unused oil. The rats were sacrificed 24 hours after the last administration, and blood and part of the skin were collected. Alkaline phosphatase (ALP), acid phosphatase (ACP), superoxide dismutase (SOD) and malondialdehyde level in the blood and skin samples collected were evaluated. Elemental analysis of the crankcase oil was also carried out. The result revealed high lead, iron and chromium levels. Blood lead concentration of rats was significantly (P<0.05) high after seven days of administration. ALP level in skin and serum increased significantly (P<0.05) with the concentration of crankcase oil. There was a significant decrease (P<0.05) in skin ACP activity while it increased significantly (P<0.05) in the serum. Similar results were observed in the SOD levels of the serum and the skin. The level increased significantly (P<0.05) in groups D-F when compared with controls. The MDA concentration of both serum and skin were significantly (P<0.05) elevated. This suggests toxic potential of used lubricating oil and its potential predisposition to cancer.
本研究的目的是调查曲轴箱油对大鼠皮肤细胞及功能完整性的影响。30只大鼠被随机分为六组，即A - F组。A组（基线对照组）接受2毫升蒸馏水。以未使用过的油为溶剂，制备2.5%、5.0%、7.5%和10.0%（体积/体积）的曲轴箱油，将2毫升不同浓度的油分别连续7天局部涂抹于C - F组。B组作为阳性对照组，接受2毫升未使用过的油。在最后一次给药24小时后处死大鼠，采集血液和部分皮肤。对采集的血液和皮肤样本中的碱性磷酸酶（ALP）、酸性磷酸酶（ACP）、超氧化物歧化酶（SOD）和丙二醛水平进行评估。同时对曲轴箱油进行元素分析。结果显示铅、铁和铬含量较高。给药7天后，大鼠血铅浓度显著升高（P<0.05）。皮肤和血清中的ALP水平随曲轴箱油浓度的增加而显著升高（P<0.05）。皮肤ACP活性显著降低（P<0.05），而血清中的ACP活性显著升高（P<0.05）。血清和皮肤中的SOD水平也观察到类似结果。与对照组相比，D - F组的SOD水平显著升高（P<0.05）。血清和皮肤中的丙二醛浓度均显著升高（P<0.05）。这表明用过的润滑油具有潜在毒性及其致癌的潜在倾向。
