臭氧治疗链脲佐菌素诱导的糖尿病大鼠视网膜的组织病理学变化及F-ERG特征
Histopathological changes in retinas and F-ERG features of streptozotocin-induced diabetic rats treated with ozone.
作者信息
Xie Ting-Yu, Li Qin, Chen Xue-Yi
机构信息
Department of Ophthalmology, the First Affiliated Hospital of Xinjiang Medical University, Urumchi 830011, Xinjiang Uygur Autonomous Region, China.
出版信息
Int J Ophthalmol. 2016 Jun 18;9(6):816-20. doi: 10.18240/ijo.2016.06.04. eCollection 2016.
AIM
To study the histopathological changes in the retina and flash electroretinogram (F-ERG) features of ozone-treated streptozotocin (STZ)-induced diabetic rats.
METHODS
Seventy male Sprague Dawley rats were grouped as follows: blank group (GB, n=10), model control group (GM, n=18), ozone group (GO3, n=19), and oxygen group (GO2, n=18). The model was induced by single intraperitoneal injection of STZ. Ozone or oxygen enteroclysm was given twice per week for 4wk. F-ERG and histopathological examinations were performed one month after treatment.
RESULTS
Under dark adaption, as compared to GB, the other groups each had differential decreases in the a-wave amplitudes (P<0.05); the latencies were delayed in GM, GO2, and GO3 rats (P<0.05). Similar results were observed under light adaption, with the exception that the a-wave of the amplitudes (F=0.28, P>0.05). There were significant differences in the apoptosis index among the groups (P<0.05). Under ozone treatment, apoptosis was decreased in GO3 as compared to GM and GO2.
CONCLUSION
Ozone administration alleviates nerve damage and reduces pathology and apoptosis in the retinas of diabetic rats.
目的
研究经臭氧处理的链脲佐菌素（STZ）诱导的糖尿病大鼠视网膜的组织病理学变化及闪光视网膜电图（F-ERG）特征。
方法
将70只雄性Sprague Dawley大鼠分为以下几组：空白组（GB，n = 10）、模型对照组（GM，n = 18）、臭氧组（GO3，n = 19）和氧气组（GO2，n = 18）。通过单次腹腔注射STZ诱导模型。每周进行两次臭氧或氧气灌肠，共4周。治疗1个月后进行F-ERG和组织病理学检查。
结果
在暗适应下，与GB组相比，其他各组的a波振幅均有不同程度降低（P < 0.05）；GM、GO2和GO3组大鼠的潜伏期延迟（P < 0.05）。在明适应下观察到类似结果，但a波振幅差异无统计学意义（F = 0.28，P > 0.05）。各组间凋亡指数存在显著差异（P < 0.05）。与GM组和GO2组相比，臭氧处理组（GO3）的凋亡减少。
结论
给予臭氧可减轻糖尿病大鼠视网膜的神经损伤，减少病理改变和细胞凋亡。
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