在糖尿病小鼠模型中，眼周注射塞来昔布和普萘洛尔对血管内皮生长因子眼内水平的影响。
Effect of periocular injection of celecoxib and propranolol on ocular level of vascular endothelial growth factor in a diabetic mouse model.
作者信息
Nassiri Saman, Houshmand Gholamreza, Feghhi Mostafa, Kheirollah Alireza, Bahadoram Mohammad, Nassiri Nariman
机构信息
Department of Ophthalmology, Infectious Ophthalmic Research Center, Schoolof Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61357-15794, Khuzestan, Iran.
Department of Pharmacology, School of Pharmacy, Ahvaz Jundishapour University of Medical Sciences, Ahvaz 61357-15794, Khuzestan, Iran.
出版信息
Int J Ophthalmol. 2016 Jun 18;9(6):821-4. doi: 10.18240/ijo.2016.06.05. eCollection 2016.
AIM
To investigate the effects of periocular injection of propranolol and celecoxib on ocular levels of vascular endothelial growth factor (VEGF) in a diabetic mouse model.
METHODS
Forty 4-6wk BALB-C male mice weighing 20-25 g were used. The study groups included: non-diabetic control (group 1), diabetic control (group 2), diabetic propranolol (group 3), and diabetic celecoxib (group 4). After induction of type 1 diabetes by streptozotocin, propranolol (10 µg) and celecoxib (200 µg dissolved in carboxymethylcellulose 0.5%) were injected periocularly. The ocular level of VEGF was measured in all the study groups using enzyme-linked immuno sorbent assay (ELISA) method.
RESULTS
Ocular VEGF level was significantly increased (1.25 fold) in the diabetic control group when compared to the non-diabetic group one week after induction with streptozotocin (P=0.002). Both periocular propranolol and celecoxib significantly reduced ocular VEGF levels (P=0.047 and P<0.001, respectively). The effect was more pronounced with celecoxib.
CONCLUSION
The periocular administration of propranolol and celecoxib can significantly reduce ocular VEGF levels in a diabetic mouse model.
目的
在糖尿病小鼠模型中研究眼周注射普萘洛尔和塞来昔布对血管内皮生长因子（VEGF）眼内水平的影响。
方法
使用40只体重20 - 25 g、4 - 6周龄的BALB - C雄性小鼠。研究组包括：非糖尿病对照组（第1组）、糖尿病对照组（第2组）、糖尿病普萘洛尔组（第3组）和糖尿病塞来昔布组（第4组）。通过链脲佐菌素诱导1型糖尿病后，眼周注射普萘洛尔（10μg）和塞来昔布（200μg溶于0.5%羧甲基纤维素）。使用酶联免疫吸附测定（ELISA）法测量所有研究组的眼内VEGF水平。
结果
与非糖尿病组相比，链脲佐菌素诱导后1周，糖尿病对照组的眼内VEGF水平显著升高（1.25倍）（P = 0.002）。眼周注射普萘洛尔和塞来昔布均显著降低眼内VEGF水平（分别为P = 0.047和P < 0.001）。塞来昔布的效果更明显。
结论
在糖尿病小鼠模型中，眼周给予普萘洛尔和塞来昔布可显著降低眼内VEGF水平。
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