Alp Mehmet Numan, Oken Ozdamar Fuad, Sargon Mustafa Fevzi, Ucaner Ahmet
Department of Ophthalmology, Numune Training and Research Hospital, Ankara 06100, Turkey.
Department of Orthopedics and Traumatology, Numune Training and Research Hospital, Ankara 06100, Turkey.
Int J Ophthalmol. 2016 Jun 18;9(6):838-42. doi: 10.18240/ijo.2016.06.08. eCollection 2016.
To investigate the effect of short-term prophylactic dose of a low molecular weight heparin (LMWH) drug on the bone healing process in an animal model simulating the osteotomy obtained in dacryocystorhinostomy.
Forty male Wistar albino rats were divided into 2 groups. Subcutaneous injections of enoxaparin 1 mg/kg (enoxaparin-treated group) and saline solution (control group) were performed once daily for 4d, beginning on the first preoperative day. The osteotomy was created at the femoral diaphysis in all animals by using a Kirschner wire. Each group was further divided into 2 subgroups depending on the timing of the second operation, 14 or 21d following initial osteotomy. Patent osteotomy area on the second and the third weeks in each group were calculated by using a computer software on digital micrographs.
The patent osteotomy areas at the second and the third weeks were significantly larger in the enoxaparin-treated group than those of the control group (P<0.001 for each time-period). In the control group, the patent osteotomy area at the third week of healing was significantly smaller than that of the second week (P=0.003), whereas there was no significant difference between these two measurements in the enoxaparin-treated group (P=0.185).
Short-term administration of enoxaparin resultes in a significant alteration in bone healing at 14 and 21d after injury. LMWHs can be regarded as promising alternative adjuvants in dacryocystorhinostomy after being evaluated with further clinical and animal studies.
在模拟泪囊鼻腔造口术截骨术的动物模型中，研究短期预防性剂量的低分子量肝素（LMWH）药物对骨愈合过程的影响。
将40只雄性Wistar白化大鼠分为2组。从术前第一天开始，每天皮下注射1mg/kg依诺肝素（依诺肝素治疗组）和生理盐水（对照组），持续4天。所有动物均使用克氏针在股骨干中段进行截骨术。根据初次截骨术后14天或21天的二次手术时间，每组进一步分为2个亚组。使用计算机软件在数字显微照片上计算每组在第二周和第三周的截骨术开放面积。
依诺肝素治疗组在第二周和第三周的截骨术开放面积明显大于对照组（每个时间段P<0.001）。在对照组中，愈合第三周的截骨术开放面积明显小于第二周（P = 0.003），而依诺肝素治疗组这两个测量值之间无显著差异（P = 0.185）。
短期给予依诺肝素会导致损伤后14天和21天的骨愈合发生显著改变。经过进一步的临床和动物研究评估后，LMWHs可被视为泪囊鼻腔造口术中一种有前景的替代辅助药物。
