来自斯里兰卡的香蕉束顶病毒分离株的分子特征及其与其他分离株的遗传关系。
Molecular characterization of banana bunchy top virus isolate from Sri Lanka and its genetic relationship with other isolates.
作者信息
Wickramaarachchi W A R T, Shankarappa K S, Rangaswamy K T, Maruthi M N, Rajapakse R G A S, Ghosh Saptarshi
机构信息
Division of Plant Pathology, Department of Agriculture, Horticulture Crops Research and Development Institute, Gannoruwa, Peradeniya, 20400 Sri Lanka.
Department of Plant Pathology, K. R. C. College of Horticulture, Arabhavi, University of Horticultural Sciences, Bagalkot, Karnataka India.
出版信息
Virusdisease. 2016 Jun;27(2):154-60. doi: 10.1007/s13337-016-0311-2. Epub 2016 Apr 4.
Bunchy top disease of banana caused by Banana bunchy top virus (BBTV, genus Babuvirus family Nanoviridae) is one of the most important constraints in production of banana in the different parts of the world. Six genomic DNA components of BBTV isolate from Kandy, Sri Lanka (BBTV-K) were amplified by polymerase chain reaction (PCR) with specific primers using total DNA extracted from banana tissues showing typical symptoms of bunchy top disease. The amplicons were of expected size of 1.0-1.1 kb, which were cloned and sequenced. Analysis of sequence data revealed the presence of six DNA components; DNA-R, DNA-U3, DNA-S, DNA-N, DNA-M and DNA-C for Sri Lanka isolate. Comparisons of sequence data of DNA components followed by the phylogenetic analysis, grouped Sri Lanka-(Kandy) isolate in the Pacific Indian Oceans (PIO) group. Sri Lanka-(Kandy) isolate of BBTV is classified a new member of PIO group based on analysis of six components of the virus.
由香蕉束顶病毒（BBTV，属于矮化病毒科纳米病毒属）引起的香蕉束顶病是世界各地香蕉生产中最重要的制约因素之一。使用从表现出典型束顶病症状的香蕉组织中提取的总DNA，通过聚合酶链反应（PCR）和特异性引物扩增了来自斯里兰卡康提的BBTV分离株的六个基因组DNA组分。扩增产物大小为预期的1.0 - 1.1 kb，将其克隆并测序。序列数据分析揭示了六个DNA组分的存在；斯里兰卡分离株的DNA-R、DNA-U3、DNA-S、DNA-N、DNA-M和DNA-C。对DNA组分的序列数据进行比较并随后进行系统发育分析后，将斯里兰卡（康提）分离株归入太平洋印度洋（PIO）组。基于对该病毒六个组分的分析，BBTV的斯里兰卡（康提）分离株被归类为PIO组的一个新成员。
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