用于从杂交瘤细胞生产完整IgG及其Fab片段的合理工艺优化数据。

Data of rational process optimization for the production of a full IgG and its Fab fragment from hybridoma cells.

作者信息

Röhm Martina, Handl Alina, König Maria, Mavoungou Chrystelle, Handrick René, Schindowski Katharina

机构信息

Institute of Applied Biotechnology, University of Applied Sciences Biberach, Hubertus-Liebrecht-Strasse 35, 88400 Biberach, Germany; University of Ulm, Faculty of Medicine, Albert-Einstein-Allee 11, 89081 Ulm, Germany.

Institute of Applied Biotechnology, University of Applied Sciences Biberach, Hubertus-Liebrecht-Strasse 35, 88400 Biberach, Germany.

出版信息

Data Brief. 2016 Jun 2;8:426-35. doi: 10.1016/j.dib.2016.05.067. eCollection 2016 Sep.

DOI:10.1016/j.dib.2016.05.067

PMID:27366780

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4910301/

Abstract

This data article focuses on the production of monoclonal antibodies (mAb) and their fragments Fab and F(ab')2. Here, we present the data of an optimization protocol to improve the product yield of a hybridoma cell process using a Design of Experiment (DoE) strategy. Furthermore, the data of the evaluated conditions were used to test feeding strategies in shake flasks. They were verified in controlled 2 L fed-batch bioreactor processes. Supplementing the culture medium with human insulin-like growth factor-I (IGF-I) and Pluronic F-68, as well as a nutrient rich additive for fed-batch, resulted in improved cell growth correlating with a 7 day elongated process time and a 4.5 fold higher product titer. Finally, a rapid Fab generation protocol and the respective data are presented using different papain digestion and a camelid anti-kappa light chain VHH affinity ligand.

摘要

本文聚焦于单克隆抗体（mAb）及其Fab和F(ab')2片段的生产。在此，我们展示了使用实验设计（DoE）策略优化杂交瘤细胞工艺以提高产品产量的优化方案数据。此外，所评估条件的数据被用于测试摇瓶中的补料策略。这些策略在2L受控补料分批生物反应器工艺中得到了验证。用人胰岛素样生长因子-I（IGF-I）和普朗尼克F-68以及一种用于补料分批的富含营养的添加剂补充培养基，导致细胞生长改善，这与延长7天的工艺时间和提高4.5倍的产品滴度相关。最后，使用不同的木瓜蛋白酶消化和骆驼科抗κ轻链VHH亲和配体展示了快速Fab生成方案及相应数据。