使用反向遗传学方法观察到埃博拉病毒马科纳变种的两种自然分离株之间的病毒适应性差异。
Virus fitness differences observed between two naturally occurring isolates of Ebola virus Makona variant using a reverse genetics approach.
作者信息
Albariño César G, Guerrero Lisa Wiggleton, Chakrabarti Ayan K, Kainulainen Markus H, Whitmer Shannon L M, Welch Stephen R, Nichol Stuart T
机构信息
Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA, USA.
Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA, USA.
出版信息
Virology. 2016 Sep;496:237-243. doi: 10.1016/j.virol.2016.06.011. Epub 2016 Jun 29.
During the large outbreak of Ebola virus disease that occurred in Western Africa from late 2013 to early 2016, several hundred Ebola virus (EBOV) genomes have been sequenced and the virus genetic drift analyzed. In a previous report, we described an efficient reverse genetics system designed to generate recombinant EBOV based on a Makona variant isolate obtained in 2014. Using this system, we characterized the replication and fitness of 2 isolates of the Makona variant. These virus isolates are nearly identical at the genetic level, but have single amino acid differences in the VP30 and L proteins. The potential effects of these differences were tested using minigenomes and recombinant viruses. The results obtained with this approach are consistent with the role of VP30 and L as components of the EBOV RNA replication machinery. Moreover, the 2 isolates exhibited clear fitness differences in competitive growth assays.
在2013年末至2016年初发生于西非的埃博拉病毒病大规模暴发期间，数百个埃博拉病毒（EBOV）基因组被测序，并对病毒的基因漂移进行了分析。在之前的一份报告中，我们描述了一种高效的反向遗传学系统，该系统旨在基于2014年获得的一株马科纳变异株分离物产生重组EBOV。利用该系统，我们对马科纳变异株的2个分离株的复制和适应性进行了表征。这些病毒分离株在基因水平上几乎相同，但在VP30和L蛋白中存在单个氨基酸差异。利用微型基因组和重组病毒对这些差异的潜在影响进行了测试。用这种方法获得的结果与VP30和L作为EBOV RNA复制机制组成部分的作用一致。此外，在竞争性生长试验中，这2个分离株表现出明显的适应性差异。
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