Wu Chen-Jiang, Bao Mei-Ling, Wang Qing, Wang Xiao-Ning, Liu Xi-Sheng, Shi Hai-Bin, Zhang Yu-Dong
Department of Radiology, the First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
Department of Pathology, the First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
J Magn Reson Imaging. 2017 Jan;45(1):291-302. doi: 10.1002/jmri.25346. Epub 2016 Jul 1.
To investigate the physiopathological effects of low- and iso-osmolar contrast media (CM) on renal function with physiologic MRI and histologic-gene examination.
Forty-eight rats underwent time-course DWI and DCE-MRI at 3.0 Tesla (T) before and 5-15 min after exposure of CM or saline (Iop.370: 370 mgI/mL iopromide; Iod.320: 320 mgI/mL iodixanol; Iod.270: 270 mgI/mL iodixanol; 4 gI/kg body weight). Intrarenal viscosity was reflected by apparent diffusion coefficient (ADC). Renal physiologies were evaluated by DCE-derived glomerular filtration rate (GFR), renal blood flow (RBF), and renal blood volume (RBV). Potential acute kidney injury (AKI) was determined by histology and the expression of kidney injury molecule 1 (Kim-1).
Iop.370 mainly increased ADC in inner-medulla (△ADC : 12.3 ± 11.1%; P < 0.001). Iod.320 and Iod.270 mainly decreased ADC in outer-medulla (△ADC ; Iod.320: 16.8 ± 7.5%; Iod.270: 18.1 ± 9.5%; P < 0.001) and inner-medulla (△ADC ; Iod.320: 28.4 ± 9.3%; Iod.270: 30.3 ± 6.3%; P < 0.001). GFR, RBF and RBV were significantly decreased by Iod.320 (△GFR: 45.5 ± 24.1%; △RBF: 44.6 ± 19.0%; △RBV: 35.2 ± 10.1%; P < 0.001) and Iod.270 (33.2 ± 19.0%; 38.1 ± 15.6%; 30.1 ± 10.1%; P < 0.001), while rarely changed by Iop.370 and saline. Formation of vacuoles and increase in Kim-1 expression was prominently detected in group of Iod.320, while rarely in Iod.270 and Iop.370.
Iso-osmolar iodixanol, given at high-dose, produced prominent AKI in nonhydrated rats. This renal dysfunction could be assessed noninvasively by physiologic MRI.
1 J. Magn. Reson. Imaging 2017;45:291-302.
通过生理磁共振成像和组织学 - 基因检查，研究低渗和等渗对比剂（CM）对肾功能的生理病理影响。
48只大鼠在注射CM或生理盐水（碘普罗胺370：370mgI/mL碘普罗胺；碘克沙醇320：320mgI/mL碘克沙醇；碘克沙醇270：270mgI/mL碘克沙醇；4gI/kg体重）之前及之后5 - 15分钟，于3.0特斯拉（T）进行时间分辨扩散加权成像（DWI）和动态对比增强磁共振成像（DCE - MRI）。表观扩散系数（ADC）反映肾内黏度。通过DCE得出的肾小球滤过率（GFR）、肾血流量（RBF）和肾血容量（RBV）评估肾脏生理功能。通过组织学和肾损伤分子1（Kim - 1）的表达确定潜在的急性肾损伤（AKI）。
碘普罗胺370主要使肾内髓质的ADC升高（ΔADC：12.3±11.1%；P < 0.001）。碘克沙醇320和碘克沙醇270主要使肾外髓质的ADC降低（ΔADC；碘克沙醇320：16.8±7.5%；碘克沙醇270：18.1±9.5%；P < 0.001）以及肾内髓质的ADC降低（ΔADC；碘克沙醇320：28.4±9.3%；碘克沙醇270：30.3±6.3%；P < 0.001）。碘克沙醇320（ΔGFR：45.5±24.1%；ΔRBF：44.6±19.0%；ΔRBV：35.2±10.1%；P < 0.001）和碘克沙醇270（33.2±19.0%；38.1±
