Lawaf Shirin, Azizi Arash, Farzad Azin, Adimi Parvaneh
Gen Dent. 2016 Jul-Aug;64(4):e1-4.
Surface treatment of porcelain is required to minimize the adhesion of microorganisms to surfaces of the restoration. This study sought to assess the effects of 3 different porcelain surface treatments on adhesion of Candida albicans. This in vitro experimental study was conducted on 60 porcelain disks (10 × 3 mm) randomly divided into 4 groups of 15. The nonglazed group received no surface treatment; specimens in the other 3 groups were glazed in the furnace, overglazed with liquid glaze, or polished using a polishing kit. The specimens were washed, sterilized, and separately incubated with 350 µL of Candida albicans suspension for 24 hours. Specimens were then rinsed for 20 seconds and shaken in 1 mL of saline solution for 1 minute, and 20 µL of this suspension was cultured in a plate and incubated at 37°C for 48 hours. Candida albicans colonies were counted to assess the number of microorganisms adhering to each disk. Data were analyzed with the Kruskal-Wallis test. Statistically significant differences were found among the 4 groups in terms of C albicans adherence (P = 0.001). The nonglazed porcelain had the highest and the overglazed porcelain had the lowest mean adherence value. No statistically significant difference was noted between glazed and polished specimens. Based on the obtained results, overglazing resulted in the least adhesion of C albicans, and polishing provided a surface as smooth as a glazed surface.
需要对瓷修复体表面进行处理，以尽量减少微生物在修复体表面的黏附。本研究旨在评估3种不同的瓷表面处理方法对白色念珠菌黏附的影响。本体外实验研究使用了60个瓷盘（10×3毫米），随机分为4组，每组15个。未上釉组未进行表面处理；其他3组的标本分别进行炉内上釉、液体釉料外上釉或使用抛光套件进行抛光。标本经清洗、灭菌后，分别与350微升白色念珠菌悬液孵育24小时。然后将标本冲洗20秒，并在1毫升盐溶液中振荡1分钟，取20微升该悬液接种于平板上，在37℃下培养48小时。通过计数白色念珠菌菌落来评估黏附在每个瓷盘上的微生物数量。数据采用Kruskal-Wallis检验进行分析。在白色念珠菌黏附方面，4组之间存在统计学显著差异（P = 0.001）。未上釉瓷盘的平均黏附值最高，外上釉瓷盘的平均黏附值最低。上釉标本和抛光标本之间未观察到统计学显著差异。根据所得结果，外上釉导致白色念珠菌的黏附最少，而抛光提供了与上釉表面一样光滑的表面。
