胎牛血清中的一种因子对鸡胚视网膜体外DNA合成的抑制作用。

Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum.

作者信息

Tesoriere G, Vento R, Calvaruso G, Schiavo M R, Giuliano M, Taibi G

机构信息

Institute of Biological Chemistry, University of Palermo, Italy.

出版信息

Brain Res Dev Brain Res. 1989 May 1;47(1):19-25. doi: 10.1016/0165-3806(89)90104-1.

DOI:10.1016/0165-3806(89)90104-1

PMID:2736764

Abstract

Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.

摘要

胎牛血清抑制鸡胚视网膜外植体中的脱氧核糖核酸（DNA）合成。抑制活性通过45%饱和硫酸铵从胎牛血清中沉淀出来，并通过Sephadex G - 100和Bio - Gel P - 60柱分离，得到一个表观分子量为7000 Da的峰。DNA抑制活性对热和酸稳定，可被二硫苏糖醇和碱性处理破坏。纯化的因子对DNA合成和胸苷激酶活性的抑制作用相似；加入孵育培养基17小时后，160 ng时可产生50%的抑制效果。