Todorova Milka, Trendafilova Antoaneta, Vitkova Antonina, Petrova Maria, Zayova Ely, Antonova Daniela
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl. 9, BG-1113 Sofia.
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Gagarin Str. 2, BG-1113 Sofia.
Chem Biodivers. 2016 Aug;13(8):976-81. doi: 10.1002/cbdv.201500307. Epub 2016 Jul 22.
The amount of sesquiterpene lactones and the lactone profile of Arnica montana L. in flowering and seed formation stages in vitro and in vivo propagated from seeds of German, Ukrainian, and Austrian origin and grown in two experimental fields were studied. It was found that in vitro propagated 2-year plants in full flowering stage accumulated higher amount of lactones in comparison to in vivo propagated 3-year plants and to the seed formation stage, respectively. Helenalins predominated in in vivo propagated 2-year or in vitro propagated 3-year plants. 2-Methylbutyrate (2MeBu) was the principal ester in the samples with prevalence of helenalins, while isobutyrate (iBu) was the major one in the samples with predominance of 11,13-dihydrohelenalins. The results revealed that the environmental conditions on Vitosha Mt. are more suitable for cultivation of A. montana giving higher content of lactones.
研究了从德国、乌克兰和奥地利种子来源的山金车在体外和体内繁殖并种植在两个试验田中的开花期和种子形成期的倍半萜内酯含量及内酯谱。结果发现，与体内繁殖的3年生植株和种子形成期相比，体外繁殖的处于盛花期的2年生植株积累了更高含量的内酯。海伦内酯在体内繁殖的2年生植株或体外繁殖的3年生植株中占主导地位。在以海伦内酯为主的样品中，2-甲基丁酸酯（2MeBu）是主要酯类，而在以11,13-二氢海伦内酯为主的样品中，异丁酸酯（iBu）是主要酯类。结果表明，维托沙山的环境条件更适合种植山金车，其内酯含量更高。
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