Minami Shohei, Kuroda Yudai, Terada Yutaka, Yonemitsu Kenzo, Van Nguyen Dung, Kuwata Ryusei, Shimoda Hiroshi, Takano Ai, Maeda Ken
Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan.
Virus Genes. 2016 Dec;52(6):858-862. doi: 10.1007/s11262-016-1365-3. Epub 2016 Jul 1.
In an epidemiological study of ferret coronaviruses (FRCoVs), novel FRCoV strains (Saitama-1 and Aichi-1) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and nucleotide sequence analysis of partial RNA-dependent RNA polymerase (RdRp) genes. Phylogenetic analysis indicated that these strains belonged to different clusters from other FRCoV strains. Next, the nucleotide sequence of the 3'-terminal region of Saitama-1 (8271 bases) strain was determined and compared with those of the other FRCoVs, indicating that the Saitama-1 strain differed from the previously reported MSU-1 and MSU-2 strains in the regions encoding spike (S) protein, nucleocapsid, and open reading frame 7b. Furthermore, the results of SimPlot analysis indicated that FRCoV (MSU-2 strain) emerged via a recombination event of S protein between the MSU-1 and Saitama-1 strains. This mechanism is similar to that responsible for the emergence of type II feline coronavirus. This information will be useful for understanding the pathogenesis of FRCoV in ferrets.
在一项关于雪貂冠状病毒（FRCoVs）的流行病学研究中，通过逆转录聚合酶链反应（RT-PCR）以及对部分依赖RNA的RNA聚合酶（RdRp）基因进行核苷酸序列分析，检测到了新型FRCoV毒株（埼玉-1和爱知-1）。系统发育分析表明，这些毒株与其他FRCoV毒株属于不同的簇。接下来，测定了埼玉-1毒株（8271个碱基）3'-末端区域的核苷酸序列，并与其他FRCoV毒株的序列进行比较，结果表明埼玉-1毒株在编码刺突（S）蛋白、核衣壳和开放阅读框7b的区域与先前报道的MSU-1和MSU-2毒株不同。此外，SimPlot分析结果表明，FRCoV（MSU-2毒株）是通过MSU-1和埼玉-1毒株之间S蛋白的重组事件产生的。这种机制与导致II型猫冠状病毒出现的机制相似。这些信息将有助于理解FRCoV在雪貂中的发病机制。
