[慢性乙肝病毒感染患者CD8(+)T细胞中Toll样受体2的表达增强]

[The enhanced expression of Toll-like receptor 2 in CD8(+)T cells of chronic HBV infected patients].

作者信息

Yun Chenxia, Xiao Jian, Kang Di, Wang Qihui, Peng Lishan, Liu Xian, Leng Jing

机构信息

Guangxi Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models, Nanning 530001; Department of Immunology and Microbiology, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China.

Department of Clinical Laboratory, Zhengzhou Children's Hospital, Zhengzhou 450000, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Jun;32(6):812-5.

PMID:27371850

Abstract

Objective To detect the level of Toll-like receptor 2 (TLR2) in CD8(+)T cells of chronic HBV infected patients without antiviral treatment. Methods Forty chronic HBV infected donors without antivirus treatment and 19 healthy donors were enrolled in our study. All donors were divided into three groups: a high viral load (HBV DNA>1×10(4) copies/mL) group, a low viral load (HBV DNA>1×10(4) copies/mL) group, and a healthy control group. After the isolation and staining of peripheral blood mononuclear cells (PBMCs), the levels of TLR2, CD38, HLA-DR, CD95, programmed death-1 (PD-1) in CD8(+)T cells were detected by flow cytometry to further analyze the correlations between TLR2 and CD38, HLA-DR, CD95, PD-1. Results The expression of TLR2 in CD8(+)T cells of the HBV infected patients were significantly higher than that in the healthy donors. The expression of TLR2 in CD8(+)T cells of the low viral load group was significantly higher than that in the high viral load group. There were no correlations between the expression of TLR2 and the expressions of CD38, HLA-DR, CD95 and PD-1 in CD8(+)T cells in the chronic HBV infected patients. Conclusion The expression of TLR2 in CD8(+)T cells increases in chronic HBV infected patients.

摘要

目的检测未经抗病毒治疗的慢性HBV感染患者CD8(+)T细胞中Toll样受体2（TLR2）水平。方法本研究纳入40例未经抗病毒治疗的慢性HBV感染供者和19例健康供者。所有供者分为三组：高病毒载量（HBV DNA>1×10(4)拷贝/mL）组、低病毒载量（HBV DNA>1×10(4)拷贝/mL）组和健康对照组。对外周血单个核细胞（PBMCs）进行分离和染色后，采用流式细胞术检测CD8(+)T细胞中TLR2、CD38、HLA-DR、CD95、程序性死亡受体1（PD-1）水平，以进一步分析TLR2与CD38、HLA-DR、CD95、PD-1之间的相关性。结果 HBV感染患者CD8(+)T细胞中TLR2表达明显高于健康供者。低病毒载量组CD8(+)T细胞中TLR2表达明显高于高病毒载量组。慢性HBV感染患者CD8(+)T细胞中TLR2表达与CD38、HLA-DR、CD95和PD-1表达之间无相关性。结论慢性HBV感染患者CD8(+)T细胞中TLR2表达增加。