Lopes Bianca Rebelo, Barreiro Juliana Cristina, Cass Quezia Bezerra
SEPARARE Núcleo de Pesquisa em Cromatografia, Departamento de Química, Universidade Federal de São Carlos, Caixa Postal 676, São Carlos 13565-905, Brazil.
SEPARARE Núcleo de Pesquisa em Cromatografia, Departamento de Química, Universidade Federal de São Carlos, Caixa Postal 676, São Carlos 13565-905, Brazil.
J Pharm Biomed Anal. 2016 Oct 25;130:318-325. doi: 10.1016/j.jpba.2016.06.012. Epub 2016 Jun 16.
An overview of bioanalytical methods for the determination of environmental and pharmaceutical contaminants in human milk is presented. The exposure of children to these contaminants through lactation has been widely investigated. The human milk contains diverse proteins, lipids, and carbohydrates and the concentration of these components is drastically altered during the lactation period providing a high degree of an analytical challenge. Sample collection and pretreatment are still considered the Achilles' heel. This review presents liquid chromatographic methods developed in the last 10 years for this complex matrix with focuses in the extraction and quantification steps. Green sample preparation protocols have been emphasized.
本文综述了用于测定母乳中环境污染物和药物污染物的生物分析方法。通过哺乳，儿童接触这些污染物的情况已得到广泛研究。母乳中含有多种蛋白质、脂质和碳水化合物，在哺乳期这些成分的浓度会发生剧烈变化，这给分析带来了很大挑战。样本采集和预处理仍然被认为是关键弱点。本综述介绍了过去10年中针对这种复杂基质开发的液相色谱方法，重点关注提取和定量步骤。文中强调了绿色样本制备方案。
