Okrasa S, Kotwica G, Ciereszko R, Dusza L, Czarnyszewicz J
Department of Animal Physiology, University of Agriculture and Technology, Olsztyn, Poland.
Exp Clin Endocrinol. 1989 Apr;93(1):95-103. doi: 10.1055/s-0029-1210842.
Blood samples were collected from 8 lactating sows every 2 h for 24 h periods on the Days 5, 10, 15, 20, 25, 30 and 35 of lactation. Additional samples were drawn from four sows during morning spontaneous sucklings on the same days. Prolactin (PRL), oxytocin (OT), estradiol 17-beta (E2) and estrone (E1) were measured by RIA. Corticosteroids (CS) were determined by competitive protein binding assay (CPBA). In 4 of 8 sows, elevated concentrations of PRL were concomitant with greater concentrations of CS and slightly larger concentrations of OT. Mean concentrations of E1 were markedly greater than those of E2 in 6 of 8 sows. A response of PRL to nursing was observed in 90% of all suckling events studied. A CS increase was observed in 64% of suckling occurrences. During suckling OT surges appeared with peripheral concentrations greater on day 5 and 10 of lactation versus days 15, 20, 25, 30 and 35.
在泌乳期的第5、10、15、20、25、30和35天，每隔2小时从8头泌乳母猪采集血样，持续24小时。在同一天的早晨自然哺乳期间，从4头母猪采集额外的血样。采用放射免疫分析法（RIA）测定催乳素（PRL）、催产素（OT）、雌二醇17-β（E2）和雌酮（E1）。采用竞争性蛋白结合分析法（CPBA）测定皮质类固醇（CS）。在8头母猪中的4头，PRL浓度升高伴随着CS浓度升高以及OT浓度略有升高。在8头母猪中的6头，E1的平均浓度显著高于E2。在所有研究的哺乳事件中，90%观察到PRL对哺乳有反应。在64%的哺乳事件中观察到CS增加。在哺乳期间，OT出现激增，泌乳第5天和第10天的外周浓度高于第15、20、25、30和35天。
