Woods J E, Scanes C G, Seeley M, Cozzi P, Onyeise F, Thommes R C
Department of Biological Sciences, DePaul University, Chicago, Illinois 60614.
Gen Comp Endocrinol. 1989 Apr;74(1):1-13. doi: 10.1016/0016-6480(89)90108-1.
Plasma luteinizing hormone (LH) concentrations and the numerical density (Nv) of gonadal LH-binding (LH-positive) cells were determined in intact male and female chick embryos from Days 10.5 through 18.5 of incubation for plasma LH and from Days 6.5 through 19.5 for LH-binding cells, as well as in Day 15.5 decapitated ("hypophysectomized") embryos with pituitary transplants for both plasma LH and LH-binding cells. The data demonstrate that LH is already present in the plasma of male and female embryos as early as Day 10.5, the first day examined. Plasma LH levels in hypophysectomized embryos were statistically significantly lower than those of intact embryos, while pituitary transplants to the chorioallantoic membrane of hypophysectomized embryos elevated plasma LH concentrations to levels not statistically different from those of intacts. The "role(s)" of plasma LH levels and changes in form and numerical density (Nv) of gonadal LH-binding cells in the maturation of the pituitary-gonadal axes of the chick embryo are discussed and evaluated.
在孵化第10.5天至18.5天的完整雄性和雌性鸡胚中测定血浆促黄体生成素（LH）浓度以及性腺LH结合（LH阳性）细胞的数值密度（Nv）（用于血浆LH），在孵化第6.5天至19.5天的鸡胚中测定（用于LH结合细胞），同时也在第15.5天断头（“垂体切除”）并进行垂体移植的胚胎中测定血浆LH和LH结合细胞。数据表明，早在第10.5天，即检测的第一天，雄性和雌性胚胎的血浆中就已经存在LH。垂体切除胚胎的血浆LH水平在统计学上显著低于完整胚胎，而将垂体移植到垂体切除胚胎的绒毛尿囊膜上可使血浆LH浓度升高至与完整胚胎无统计学差异的水平。本文讨论并评估了血浆LH水平以及性腺LH结合细胞形态和数值密度（Nv）变化在鸡胚垂体 - 性腺轴成熟中的“作用”。
