Cherny V V, Paulitschke M, Simonova M V, Hessel E, Sokolov V S, Lerche D, Markin V S
A.N. Frumkin Institute of Electrochemistry, Academy of Sciences, Moscow, USSR.
Gen Physiol Biophys. 1989 Feb;8(1):23-37.
The action of the antiviral drug rimantadine on the structure of bilayer lipid membranes (BLM) and RBC membranes was investigated. Structural changes in BLM were recorded by ionophore conductivity changes and by changes in the third harmonic of capacity current signal due to lateral compression of BLM in an electric field. It was shown that the adsorption of rimantadine on BLM results in an increase in ionophore mobility in bilayer membranes of dioleolyllecithin (DOL) and common lipids of bovine brain (CL) and in a decrease in those of azolectin (A). Relative changes in the third harmonic signal also depend on the membrane composition and have different signs. The results may be explained by the rimantadine action on the lipid bilayer structure: "rigidification" of A-membranes and "fluidization" of BLM from DOL and CL. Structural reorganization of RBC membranes as investigated by the ability of the cells to enter a micropipette (inner diameter greater than or equal to 3 microns) thereby undergoing deformation. It was shown that rimantadine influences RBC deformability due to drug induced inhomogenous mechanical membrane properties. Also, rimantadine accelerated the process of artificially induced aggregation of erythrocytes. The relation of the effects on artificial and biological membranes, and the structural changes in the lipid phase of membrane are discussed.
研究了抗病毒药物金刚乙胺对双层脂质膜（BLM）和红细胞膜结构的作用。通过离子载体电导率变化以及由于电场中BLM的横向压缩导致的电容电流信号三次谐波变化来记录BLM的结构变化。结果表明，金刚乙胺在BLM上的吸附导致离子载体在二油酰卵磷脂（DOL）双层膜和牛脑普通脂质（CL）中的流动性增加，而在偶氮卵磷脂（A）中的流动性降低。三次谐波信号的相对变化也取决于膜的组成，并且具有不同的符号。这些结果可以通过金刚乙胺对脂质双层结构的作用来解释：A膜的“刚性化”以及DOL和CL的BLM的“流化”。通过细胞进入微吸管（内径大于或等于3微米）从而发生变形的能力来研究红细胞膜的结构重组。结果表明，金刚乙胺由于药物诱导的膜机械性能不均匀而影响红细胞的变形能力。此外，金刚乙胺加速了人工诱导的红细胞聚集过程。讨论了对人工膜和生物膜的影响以及膜脂质相的结构变化之间的关系。
