Sen A, Khetarpal Sm A, Jetley S
Classified Specialist (Pathology & Micro), Army Hospital (R&R), Delhi Cantt-10.
Consultant (Pathology), Army Hospital (R&R), Delhi Cantt-10.
Med J Armed Forces India. 2010 Apr;66(2):142-6. doi: 10.1016/S0377-1237(10)80127-3. Epub 2011 Jul 21.
Removal of leucocytes from cellular blood components is associated with reduction of several transfusion associated adverse reactions.
A total of 400 units of packed red blood cells (RBCs) were subjected to leucodepletion at room temperature and 4°C using different commercially available prestorage and bedside filters (Terumo Penpol Immugard III and Pall Medical BPF-4). Pre-filtration and post-filtration parameters were compared to assess the efficacy of prestorage leucodepletion vis-à-vis bedside leucodepletion and the requirement of universal leucodepletion.
Mean post-filtration red cell recovery ranged from 88.49-93.49% with all bags showing more than 85% red cell recovery. Mean post-filtration residual leucocyte count ranged from 0.205 × 10(6)-0.338 × 10(6)/bag with all bags showing more than log 3 leucoreduction. Prestorage leucoreduction achieved by the polyurethane filter was better than that achieved by the polyester filter. Red cell recovery with the bedside filters at room temperature was significantly less than that with prestorage filters at either temperature.
This study suggests that prestorage leucoreduction is preferable over bedside leucoreduction and that polyurethane filters are better than polyester filters since leucodepletion achieved with the former is higher. We recommend selective log 3 leucodepletion using polyurethane prestorage filters for patients with specific indications.
从细胞血液成分中去除白细胞与减少多种输血相关不良反应有关。
使用不同的市售前储存和床边过滤器（Terumo Penpol Immugard III和颇尔医疗BPF-4），在室温及4°C条件下对总共400单位的浓缩红细胞（RBC）进行白细胞去除处理。比较过滤前和过滤后的参数，以评估前储存白细胞去除相对于床边白细胞去除的效果以及普遍白细胞去除的必要性。
所有袋子的过滤后红细胞平均回收率在88.49%至93.49%之间，所有袋子的红细胞回收率均超过85%。过滤后平均残留白细胞计数在0.205×10⁶至0.338×10⁶/袋之间，所有袋子的白细胞减少率均超过对数3。聚氨酯过滤器实现的前储存白细胞减少效果优于聚酯过滤器。室温下床边过滤器的红细胞回收率明显低于任一温度下前储存过滤器的红细胞回收率。
本研究表明，前储存白细胞减少优于床边白细胞减少，并且聚氨酯过滤器优于聚酯过滤器，因为前者实现的白细胞去除率更高。我们建议对有特定适应症的患者使用聚氨酯前储存过滤器进行选择性对数3白细胞去除。
