Deng Zhi, Chen Jiangshu, Leclercq Julie, Zhou Zhuangzhi, Liu Changren, Liu Hui, Yang Hong, Montoro Pascal, Xia Zhihui, Li Dejun
Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences Danzhou, China.
Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural SciencesDanzhou, China; Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan UniversityHaikou, China.
Front Plant Sci. 2016 Jun 7;7:789. doi: 10.3389/fpls.2016.00789. eCollection 2016.
As a highly conserved protein, the translationally controlled tumor protein (TCTP) carries out vital roles in various life processes. In rubber tree, two TCTP genes, HbTCTP and HbTCTP1, were cloned, but only HbTCTP1 was studied in details. In this study, cis-acting regulatory elements, expression patterns, subcellular localization, interacting proteins, and antioxidant activity of HbTCTP were systematically analyzed. Besides the common cis-acting regulatory elements, HbTCTP promoter also harbored various known cis-elements that respond to hormone/stresses. Being consistent with the aforementioned results, HbTCTP was regulated by drought, low temperature, high salt, ethylene (ET), wounding, H2O2, and methyl jasmonate (MeJA) treatments. HbTCTP was expressed throughout different tissues and developmental stages of leaves. In addition, HbTCTP was associated with tapping panel dryness (TPD). HbTCTP was localized in the membrane, cytoplasm and the nucleus, and interacted with four proteins rubber elongation factor (REF), 17.5 kDa heat shock family protein, annexin, and REF-like stress related protein 1. Being similar to HbTCTP1, HbTCTP also indicated antioxidant activity in metal-catalyzed oxidation (MCO) system. Our results are useful for further understanding the molecular characterization and expression profiles of HbTCTP, but also lay a solid foundation for elucidating the function of HbTCTP in rubber tree.
作为一种高度保守的蛋白质，翻译调控肿瘤蛋白（TCTP）在各种生命过程中发挥着重要作用。在橡胶树中，克隆了两个TCTP基因，即HbTCTP和HbTCTP1，但仅对HbTCTP1进行了详细研究。在本研究中，系统分析了HbTCTP的顺式作用调控元件、表达模式、亚细胞定位、相互作用蛋白和抗氧化活性。除了常见的顺式作用调控元件外，HbTCTP启动子还含有各种已知的响应激素/胁迫的顺式元件。与上述结果一致，HbTCTP受干旱、低温、高盐、乙烯（ET）、创伤、H2O2和茉莉酸甲酯（MeJA）处理的调控。HbTCTP在叶片的不同组织和发育阶段均有表达。此外，HbTCTP与割面干燥症（TPD）有关。HbTCTP定位于细胞膜、细胞质和细胞核，并与四种蛋白质相互作用，即橡胶延伸因子（REF）、17.5 kDa热休克家族蛋白、膜联蛋白和REF样胁迫相关蛋白1。与HbTCTP1相似，HbTCTP在金属催化氧化（MCO）系统中也表现出抗氧化活性。我们的结果不仅有助于进一步了解HbTCTP的分子特征和表达谱，也为阐明HbTCTP在橡胶树中的功能奠定了坚实的基础。
