Rezai H, Ardehali S, Teplitz R L
Department of Medical Pathology, School of Medicine, University of California, Davis 95616.
Immunol Invest. 1989 Jun;18(5):671-88. doi: 10.3109/08820138909057754.
The interaction of the immune complex (IC) composed of DNA and monoclonal anti-DNA antibody with thioglycollate-stimulated mouse peritoneal macrophages was investigated. The immune complex: macrophage interaction was shown to be highly time and temperature dependent; at 37 degrees C it proceeds faster than at 0 degrees C, although there is higher overall binding of IC to macrophages at 0 degrees C. The maximum bound IC detected was at a DNA/antibody ratio of 6.2ng/ml to 7.3 micrograms/ml. Higher densities of either DNA or antibody inhibited IC: phagocyte interaction. Binding of the IC to macrophages is through cell surface Fc receptors and is enhanced in the presence of 40 mg/ml albumin. Fresh human and mouse sera at the concentration of 10 percent, inhibited the IC binding to mouse peritoneal macrophages. Macrophage receptors for IC are not saturated even after 60 minutes. Addition of either chloroquine or cytochalasin B, resulted in increased binding of IC to macrophages.
研究了由DNA和单克隆抗DNA抗体组成的免疫复合物（IC）与巯基乙酸盐刺激的小鼠腹腔巨噬细胞的相互作用。免疫复合物与巨噬细胞的相互作用显示出高度的时间和温度依赖性；在37℃时比在0℃时进行得更快，尽管在0℃时IC与巨噬细胞的总体结合更高。检测到的最大结合IC是在DNA/抗体比例为6.2纳克/毫升至7.3微克/毫升时。DNA或抗体的更高密度会抑制IC与吞噬细胞的相互作用。IC与巨噬细胞的结合是通过细胞表面Fc受体，并且在存在40毫克/毫升白蛋白的情况下会增强。浓度为10%的新鲜人血清和小鼠血清会抑制IC与小鼠腹腔巨噬细胞的结合。即使在60分钟后，巨噬细胞对IC的受体也未饱和。添加氯喹或细胞松弛素B会导致IC与巨噬细胞的结合增加。
