Dhume R A, Nagwekar M D, Noronha A, Varde M R, Mascarenhas J F
Department of Physiology, Goa Medical College, Santa Cruz.
Indian J Physiol Pharmacol. 1989 Jan-Mar;33(1):1-9.
Place learning behaviour for working (short term) memory and reference (long term) memory is studied with the Four-arm radial open maze (FAROM) in 18 rats divided equally in three groups. In group I, 0.5 mg of atropine was injected intra-peritoneally 30 minutes before the trial. In group II, saline and in group III Glycopyrrolate were injected instead. Twenty three hours hungry animals were tested on each day in the maze to search for food kept in one of the eight cul-de-sacs of maze. The latency i.e. the time to reach the goal cul-de-sacs, as well as the error score i.e. the number of entries in the non-goal cul-de-sacs were counted during six consecutive trials, per day. Each trial duration was 5 minutes or the time taken by the animal to search the goal compartment whichever was less. The inter-trials period was 10 min and the work was carried out for a period of 3 weeks. The results show that atropine does block effectively both the memory faculties i.e. working and reference memory and that level of memory deficit induced by atropine is related to the rate of drug uptake by the central cholinergic receptors.
采用四臂放射状开放迷宫（FAROM）对18只大鼠进行位置学习行为研究，以探究工作（短期）记忆和参照（长期）记忆，这些大鼠被平均分为三组。在第一组中，试验前30分钟腹腔注射0.5毫克阿托品。在第二组中，注射生理盐水，在第三组中则注射格隆溴铵。每天对禁食23小时的动物在迷宫中进行测试，以寻找藏在迷宫八个死胡同之一中的食物。在每天连续的六次试验中，记录潜伏期（即到达目标死胡同的时间）以及错误分数（即进入非目标死胡同的次数）。每次试验持续时间为5分钟或动物找到目标隔室所需的时间，以较短者为准。试验间隔为10分钟，实验持续3周。结果表明，阿托品确实能有效阻断工作记忆和参照记忆这两种记忆能力，且阿托品引起的记忆缺陷程度与中枢胆碱能受体对药物的摄取率有关。
