Saiga T, Kashu I, Tabuchi K, Midorikawa O
Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
Invasion Metastasis. 1989;9(4):254-68.
The correlation between the growth of ML2, ML3 and ML4 cell lines in 1% agar cultures and their metastatic potential (mouse lung adenocarcinoma) was investigated. The growth ability in 1% agar cultures of ML2, ML3 and ML4 correlated far better with growth rates at lung metastatic sites than with the period required for the cells to reach the lungs following transplantation into the subcutaneum. Clones from 1% agar cultures of ML2, ML3 and ML4 show more conspicuous invasive and destructive growth than their monolayer counterparts in the muscle organ culture assay. We discuss the possible significance of 1% agar cultures to the investigation of 'contraenvironmental-pressure cell division capability', an important factor in the invasive and metastatic potential of cancer.
研究了ML2、ML3和ML4细胞系在1%琼脂培养物中的生长与其转移潜能（小鼠肺腺癌）之间的相关性。ML2、ML3和ML4在1%琼脂培养物中的生长能力与肺转移部位的生长速率相关性远高于与细胞皮下移植后到达肺部所需时间的相关性。在肌肉器官培养试验中，来自ML2、ML3和ML4的1%琼脂培养物的克隆比其单层对应物表现出更明显的侵袭性和破坏性生长。我们讨论了1%琼脂培养物对于研究“抗环境压力细胞分裂能力”的可能意义，这是癌症侵袭和转移潜能的一个重要因素。
