Yamaguchi T, Akamatsu N, Hamada Y, Sato H, Toshima T, Hagino T
Department of Orthopaedic Surgery, Yamanashi Medical College, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1989 Apr;63(4):296-307.
The vascularization of fresh whole knee and hip joints was done as part of an allograft transplant procedure involving forty rats (12 weeks old). Cyclosporin and prednisolone were injected into thirty-five recipient rats; five rats did not receive any drugs and were used as the control group. All control group rats with necrotic limbs died two to three weeks after the operation. Eleven of the twenty rats with transplanted knee joints and ten of the fifteen rats with transplanted hip joints began walking with full weight-bearing and the transplanted joints exhibited good mobility one month after the operation. Autopsy findings four, eight and sixteen weeks after the operation showed no degenerative or necrotic changes of the articular cartilage or subchondral bone of the joints. We concluded that this technique of vascularized whole-joint transplantation in rats was successful. This may be clinically applicable to reconstructive surgery after resection of diseased joints.
对40只（12周龄）大鼠进行同种异体移植手术，其中包括新鲜全膝关节和髋关节的血管化处理。向35只受体大鼠注射环孢素和泼尼松龙；5只大鼠未接受任何药物，用作对照组。所有对照组中出现肢体坏死的大鼠在术后两到三周死亡。术后一个月，20只接受膝关节移植的大鼠中有11只、15只接受髋关节移植的大鼠中有10只开始完全负重行走，移植关节活动良好。术后4周、8周和16周的尸检结果显示，关节软骨或软骨下骨无退行性或坏死性改变。我们得出结论，大鼠血管化全关节移植技术是成功的。这在临床上可能适用于病变关节切除后的重建手术。
