[在贝宁作为预防母婴传播一部分的情况下，利用干血斑对感染艾滋病毒母亲所生儿童进行艾滋病毒-1感染早期诊断]

[Use of dried blood spots in early diagnosis of HIV-1 infection in children born to HIV-infected mothers as part of the prevention of mother-to-child transmission in Benin].

作者信息

Tchiakpe E, Hounto-Ogouyemi A, Diop Ndiaye H, Diouara A A M, Aïssi A K, Keke R K, Kpangon A A, Lafia B, Métadokou D, Bouraïma B, Anthony D, Hounsinou A, Alao M J, Azondekon A, Ahouidi A D, Bei A K, Mbengue M A S, Touré Kane C, Zannou D M

机构信息

Laboratoire de référence du Programme national de lutte contre le sida, direction nationale de la Santé publique, ministère de la Santé, 04 BP 378, Cotonou, Bénin.

Laboratoire de bactériologie et de virologie, université Cheikh-Anta-Diop, BP 7325, 30, av Pasteur, Dakar, Sénégal.

出版信息

Bull Soc Pathol Exot. 2016 Aug;109(3):155-9. doi: 10.1007/s13149-016-0509-y. Epub 2016 Jul 7.

DOI:10.1007/s13149-016-0509-y

PMID:27385037

Abstract

The goal of this study was to evaluate using the molecular diagnosis, infection transmission rate of HIV in children born to HIV-1 positive mothers as part of the prevention of mother-to-child transmission (PMTCT) in Benin. The sample consisted of 524 dried blood spots (DBS) of children born to HIV-1 positive mothers, from 30 sites (PMTCT) taken between October 2009 and June 2010. The diagnosis of HIV-1 was performed by the qualitative detection of viral nucleic acids (RNA and DNA) in DBS on filter paper using the Abbott RealTime(®) HIV-1 Qualitative assay. We found that 51 DBS were positive (9.7%) and 473 were negative (90.3%). The failure rate of PMTCT among 420 mothers who received antiretroviral prophylaxis was 6.7% (28/420). This failure rate was significantly higher among children born to infected mothers on antiretroviral monotherapy than on triple therapy (HAART). The results of our study enrich the data in the literature on highly active antiretroviral chemoprophylaxis to reduce the transmission of HIV-1 from mother to child.

摘要

本研究的目的是通过分子诊断评估在贝宁作为预防母婴传播（PMTCT）一部分的HIV-1阳性母亲所生儿童中的HIV感染传播率。样本包括2009年10月至2010年6月期间从30个地点（PMTCT）采集的524份HIV-1阳性母亲所生儿童的干血斑（DBS）。使用雅培实时（®）HIV-1定性检测法通过对滤纸上DBS中的病毒核酸（RNA和DNA）进行定性检测来诊断HIV-1。我们发现51份DBS呈阳性（9.7%），473份呈阴性（90.3%）。在接受抗逆转录病毒预防的420名母亲中，PMTCT的失败率为6.7%（28/420）。在接受抗逆转录病毒单药治疗的感染母亲所生儿童中，这一失败率显著高于接受三联疗法（HAART）的儿童。我们的研究结果丰富了关于高效抗逆转录病毒化学预防以减少HIV-1母婴传播的文献数据。