Natarajan Saravanan, Bhuvaneshwari M, Lakshmi D Shanthana, Mrudula P, Chandrasekaran N, Mukherjee Amitava
Centre for Nanobiotechnology, VIT University, Vellore, 632 014, India.
Reverse Osmosis Membrane Division, CSIR-Central Salt and Marine Chemicals Research Institute 10 (CSIR-CSMCRI), Council of Scientific and Industrial Research (CSIR), G. B. Marg, 11, Bhavnagar, 364 002, Gujarat, India.
Environ Sci Pollut Res Int. 2016 Oct;23(19):19529-40. doi: 10.1007/s11356-016-7102-6. Epub 2016 Jul 7.
TiO2 and Ag NPs are widely used as antibacterial agents against many bacterial pathogens. Chitosan (polymer) itself acts as a strong antibacterial agent. Hence, chitosan/TiO2/Ag NPs incorporated nanocomposite film was prepared against packed drinking water bacterial strains. A concentration-dependent increase in the reduction of cell viability was observed in all the isolates under UV-C and dark exposure conditions. The bacteria consortium showed greater resistance against antibacterial effects of chitosan/TiO2/Ag nanocomposite as compared to single isolates. Glycocalyx test and mass assessment conclude the effective antibacterial activity by inhibiting bacterial adhesion on the film surface. The release of LDH and generation of ROS act as the predominant antibacterial mechanism induced by TiO2/Ag NPs. Surface characterization of chitosan/TiO2/Ag nanocomposite was studied by FTIR and XRD analyses and SEM analysis after interaction with the bacteria.
二氧化钛（TiO₂）和银纳米颗粒（Ag NPs）被广泛用作针对多种细菌病原体的抗菌剂。壳聚糖（聚合物）本身就是一种强效抗菌剂。因此，制备了壳聚糖/TiO₂/Ag NPs复合纳米复合膜，用于对抗包装饮用水中的细菌菌株。在紫外线C（UV-C）和黑暗暴露条件下，所有分离菌株的细胞活力降低均呈现浓度依赖性增加。与单一分离菌株相比，细菌群落对壳聚糖/TiO₂/Ag纳米复合材料的抗菌作用表现出更大的抗性。糖萼测试和质量评估通过抑制细菌在膜表面的粘附来证实其有效的抗菌活性。乳酸脱氢酶（LDH）的释放和活性氧（ROS）的产生是TiO₂/Ag NPs诱导的主要抗菌机制。通过傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）分析以及与细菌相互作用后的扫描电子显微镜（SEM）分析，对壳聚糖/TiO₂/Ag纳米复合材料进行了表面表征。
